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undiluted 0.715 1:4 dilution ratio 0.447 1:9 dilution ratio 0.321 a. Calculate the slope, y-intercept, and linearity constant for the correct absorbance vs. concentration linear plot. b. Determine the actual or original protein concentration of the sample in g/mL.

undiluted 0.715 1:4 dilution ratio 0.447 1:9 dilution ratio 0.321 a. Calculate the slope, y-intercept, and linearity constant for the correct absorbance vs. concentration linear plot. b. Determine the actual or original protein concentration of the sample in g/mL.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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6) The protein content of an unknown sample was determined using bovine serum albumin (BSA) as standard. The absorbance values of the standard solutions and of the samples are as follows. BSA concentration Absorbance (ug/mL) 0.005 0.00 0.063 0.20 0.121 0.40 0.276 0.60 0.336 0.80 0.406 1.00 0.449 1.20
Transcribed Image Text:6) The protein content of an unknown sample was determined using bovine serum albumin (BSA) as standard. The absorbance values of the standard solutions and of the samples are as follows. BSA concentration Absorbance (ug/mL) 0.005 0.00 0.063 0.20 0.121 0.40 0.276 0.60 0.336 0.80 0.406 1.00 0.449 1.20
Samples Absorbance undiluted 0.715 1:4 dilution ratio 0.447 1:9 dilution ratio 0.321 a. Calculate the slope, y-intercept, and linearity constant for the correct absorbance vs, concentration linear plot. b. Determine the actual or original protein concentration of the sample in ug/mL.
Transcribed Image Text:Samples Absorbance undiluted 0.715 1:4 dilution ratio 0.447 1:9 dilution ratio 0.321 a. Calculate the slope, y-intercept, and linearity constant for the correct absorbance vs, concentration linear plot. b. Determine the actual or original protein concentration of the sample in ug/mL.
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