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2. Usé thè linear regression équation ànd the fóllowing Abs 595 dată and dilution factors för calculating your protein concentrations: SAMPLE ABS 595 DILUTION Calculated FACTOR [protein] (mg/mL) 3.098 CRUDE 0.114 0.119 0.176 10 FLOW THRU 5 PRE DESALTING 3 POST DESALTING 0.157 3

2. Usé thè linear regression équation ànd the fóllowing Abs 595 dată and dilution factors för calculating your protein concentrations: SAMPLE ABS 595 DILUTION Calculated FACTOR [protein] (mg/mL) 3.098 CRUDE 0.114 0.119 0.176 10 FLOW THRU 5 PRE DESALTING 3 POST DESALTING 0.157 3

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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1. Use the following Absorbance Data to generate a Bradford Assay Standard Curve. Protein Concentration Absorbance at 595nm (mg/mL) 0.05 Chart Title 0.0092 0.1 0.02 0.2 0.04 0.3 0.112 0.4 0.167 0.5 0.1980 0.6 0.2436 Absorbance at 595nm 0.3 4y = 0.4499x - 0.0254 R? = 0.9824 0.25 0.2 0.15 0.1 0.05 0.1 0.2 0,3 0.4 0,5 0.6 0.7
Transcribed Image Text:1. Use the following Absorbance Data to generate a Bradford Assay Standard Curve. Protein Concentration Absorbance at 595nm (mg/mL) 0.05 Chart Title 0.0092 0.1 0.02 0.2 0.04 0.3 0.112 0.4 0.167 0.5 0.1980 0.6 0.2436 Absorbance at 595nm 0.3 4y = 0.4499x - 0.0254 R? = 0.9824 0.25 0.2 0.15 0.1 0.05 0.1 0.2 0,3 0.4 0,5 0.6 0.7
2. Use the linear regression equation and the following Abs 595 data and dilution factors for calculating your protein concentrations: SAMPLE ABS 595 DILUTION Calculated FACTOR [protein] (mg/mL) CRUDE 0.114 10 3.098 FLOW THRU 0.119 5 PRE DESALTING 0.176 3 POST DESALTING 0.157 3 3. Using the protein concentrations that you calculated, determine the volume in ul of each sampl that would be needed to load 25ug of that sample on an SDS-PAGE. See example in the first row of the table. SAMPLE Volume of Vol of 4x Volume of Final total volume (ul) sample for 25ug (uL) gel loading buffer Tris for sample CRUDE 8 25 17 50 FLOW THRU 50 PRE 50 DESALTING POST 50 DESALTING
Transcribed Image Text:2. Use the linear regression equation and the following Abs 595 data and dilution factors for calculating your protein concentrations: SAMPLE ABS 595 DILUTION Calculated FACTOR [protein] (mg/mL) CRUDE 0.114 10 3.098 FLOW THRU 0.119 5 PRE DESALTING 0.176 3 POST DESALTING 0.157 3 3. Using the protein concentrations that you calculated, determine the volume in ul of each sampl that would be needed to load 25ug of that sample on an SDS-PAGE. See example in the first row of the table. SAMPLE Volume of Vol of 4x Volume of Final total volume (ul) sample for 25ug (uL) gel loading buffer Tris for sample CRUDE 8 25 17 50 FLOW THRU 50 PRE 50 DESALTING POST 50 DESALTING
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