1. Use the following Absorbance Data to generate a Bradford Assay Standard Curve.Protein ConcentrationAbsorbance at 595nm(mg/mL)0.05Chart Title0.00920.10.020.20.040.30.1120.40.1670.50.19800.60.2436Absorbance at 595nm0.34y = 0.4499x - 0.0254R? = 0.98240.250.20.150.10.050.10.20,30.40,50.60.7 2. Use the linear regression equation and the following Abs 595 data and dilution factors forcalculating your protein concentrations:SAMPLEABS 595DILUTIONCalculatedFACTOR[protein](mg/mL)CRUDE0.114103.098FLOW THRU0.1195PRE DESALTING0.1763POST DESALTING0.15733. Using the protein concentrations that you calculated, determine the volume in ul of each samplthat would be needed to load 25ug of that sample on an SDS-PAGE. See example in the first row ofthe table.SAMPLEVolume ofVol of 4xVolume ofFinal totalvolume (ul)sample for25ug (uL)gelloadingbufferTris forsampleCRUDE8251750FLOW THRU50PRE50DESALTINGPOST50DESALTING

Answered: Image /qna-images/answer/6a90c364-2dc0-42b5-af44-78e309c447a0.jpg

2. Usé thè linear regression équation ànd the fóllowing Abs 595 dată and dilution factors för calculating your protein concentrations: SAMPLE ABS 595 DILUTION Calculated FACTOR [protein] (mg/mL) 3.098 CRUDE 0.114 0.119 0.176 10 FLOW THRU 5 PRE DESALTING 3 POST DESALTING 0.157 3

2. Usé thè linear regression équation ànd the fóllowing Abs 595 dată and dilution factors för calculating your protein concentrations: SAMPLE ABS 595 DILUTION Calculated FACTOR [protein] (mg/mL) 3.098 CRUDE 0.114 0.119 0.176 10 FLOW THRU 5 PRE DESALTING 3 POST DESALTING 0.157 3

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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OWLV2 | Online teaching and X 0mySigTau xb Login | bartleby X M COMM.1113: FUND OF ORA X G what food has c - Google Se X + om/ilrm/takeAssignment/takeCovalentActivity.do?locator=assignment-take D [References] hotogray lenses incorporate small amounts of silver chloride in the glass of the lens. When light hits the AgCl particles, the following reaction occurs: hv AgCl Ag + Cl The silver metal that is formed causes the lenses to darken. The enthalpy change for this reaction is 2.90 × 10² kJ/mol. Assuming all this energy must be supplied by light, what is the maximum vavelength of light that can cause this reaction? Wavelength %3D nm Submit Answer Try Another Version 3 item attempts remaining Previous Next