Table 1: The result for test tube I to 8 using of BSA stock solution. Tube BSA Cone H;O (ml) BSA (ml) Biuret ABS mg/ml reagent (ml) 1. 1.0 4. 2. 3. 0.9 0.1 4. 0.003 3. 2. 0.8 0.2 4. 0.012 4. 3. 0.7 0.3 4. 0.021 5. 4. 0.6 0.4 0.028 6. 0.5 0.5 4 0.039 7. 6. 0.4 0.6 4. 0.042 8. 7. 0.3 0.7 4. 0.052 Table 2: The result for test tube 9 to 12 using of Unknown solution Protein Tube Uknown (ml) H;0 (ml) Biuret reagent ABS concentration (mg/ml) (ml) 9. 4. 0.036 10. 4. 0.035 11. 0.5 0.5 0.017 12. 0.5 0.5 0.016 In order to determine the actual concentration of protein in the unknown samples it is necessary to draw the standard curve (concentration on the x-axis and on the absorbance y- axis) and interpolate the absorbance values of the unknowns. Draw graph and find protein concentration(mg/ml)

Table 1: The result for test tube I to 8 using of BSA stock solution. Tube BSA Cone H;O (ml) BSA (ml) Biuret ABS mg/ml reagent (ml) 1. 1.0 4. 2. 3. 0.9 0.1 4. 0.003 3. 2. 0.8 0.2 4. 0.012 4. 3. 0.7 0.3 4. 0.021 5. 4. 0.6 0.4 0.028 6. 0.5 0.5 4 0.039 7. 6. 0.4 0.6 4. 0.042 8. 7. 0.3 0.7 4. 0.052 Table 2: The result for test tube 9 to 12 using of Unknown solution Protein Tube Uknown (ml) H;0 (ml) Biuret reagent ABS concentration (mg/ml) (ml) 9. 4. 0.036 10. 4. 0.035 11. 0.5 0.5 0.017 12. 0.5 0.5 0.016 In order to determine the actual concentration of protein in the unknown samples it is necessary to draw the standard curve (concentration on the x-axis and on the absorbance y- axis) and interpolate the absorbance values of the unknowns. Draw graph and find protein concentration(mg/ml)

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

Table 1: The result for test tube 1 to 8 using of BSA stock solution.
Tube
BSA Cone
H;O (ml)
BSA (ml)
Biuret
ABS
mg/ml
reagent (ml)
1.
1.0
4.
0.
2.
0.9
0.1
4.
0.003
3.
2.
0.8
0.2
4.
0.012
4.
3.
0.7
0.3
4.
0.021
5.
4.
0.6
0.4
4.
0.028
6.
0.5
0.5
4.
0.039
7.
6.
0.4
4.
0.042
8.
7.
0.3
0.7
4.
0.052
Table 2: The result for test tube 9 to 12 using of Unknown solution
Protein
Tube
Uknown (ml)
H;0 (ml)
Biuret reagent
ABS
concentration
(mg/ml)
(ml)
9.
4.
0.036
10.
4.
0.035
11.
0.5
0.5
4.
0.017
12.
0.5
0.5
4
0.016
In order to determine the actual concentration of protein in the unknown samples it is
necessary to draw the standard curve (concentration on the x-axis and on the absorbance y-
axis) and interpolate the absorbance values of the unknowns. Draw graph and find protein
concentration(mg/ml)

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