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Table 1: The result for test tube I to 8 using of BSA stock solution. Tube BSA Cone H;O (ml) BSA (ml) Biuret ABS mg/ml reagent (ml) 1. 1.0 4. 2. 3. 0.9 0.1 4. 0.003 3. 2. 0.8 0.2 4. 0.012 4. 3. 0.7 0.3 4. 0.021 5. 4. 0.6 0.4 0.028 6. 0.5 0.5 4 0.039 7. 6. 0.4 0.6 4. 0.042 8. 7. 0.3 0.7 4. 0.052 Table 2: The result for test tube 9 to 12 using of Unknown solution Protein Tube Uknown (ml) H;0 (ml) Biuret reagent ABS concentration (mg/ml) (ml) 9. 4. 0.036 10. 4. 0.035 11. 0.5 0.5 0.017 12. 0.5 0.5 0.016 In order to determine the actual concentration of protein in the unknown samples it is necessary to draw the standard curve (concentration on the x-axis and on the absorbance y- axis) and interpolate the absorbance values of the unknowns. Draw graph and find protein concentration(mg/ml)

Table 1: The result for test tube I to 8 using of BSA stock solution. Tube BSA Cone H;O (ml) BSA (ml) Biuret ABS mg/ml reagent (ml) 1. 1.0 4. 2. 3. 0.9 0.1 4. 0.003 3. 2. 0.8 0.2 4. 0.012 4. 3. 0.7 0.3 4. 0.021 5. 4. 0.6 0.4 0.028 6. 0.5 0.5 4 0.039 7. 6. 0.4 0.6 4. 0.042 8. 7. 0.3 0.7 4. 0.052 Table 2: The result for test tube 9 to 12 using of Unknown solution Protein Tube Uknown (ml) H;0 (ml) Biuret reagent ABS concentration (mg/ml) (ml) 9. 4. 0.036 10. 4. 0.035 11. 0.5 0.5 0.017 12. 0.5 0.5 0.016 In order to determine the actual concentration of protein in the unknown samples it is necessary to draw the standard curve (concentration on the x-axis and on the absorbance y- axis) and interpolate the absorbance values of the unknowns. Draw graph and find protein concentration(mg/ml)

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Table 1: The result for test tube 1 to 8 using of BSA stock solution. Tube BSA Cone H;O (ml) BSA (ml) Biuret ABS mg/ml reagent (ml) 1. 1.0 4. 0. 2. 0.9 0.1 4. 0.003 3. 2. 0.8 0.2 4. 0.012 4. 3. 0.7 0.3 4. 0.021 5. 4. 0.6 0.4 4. 0.028 6. 0.5 0.5 4. 0.039 7. 6. 0.4 4. 0.042 8. 7. 0.3 0.7 4. 0.052 Table 2: The result for test tube 9 to 12 using of Unknown solution Protein Tube Uknown (ml) H;0 (ml) Biuret reagent ABS concentration (mg/ml) (ml) 9. 4. 0.036 10. 4. 0.035 11. 0.5 0.5 4. 0.017 12. 0.5 0.5 4 0.016 In order to determine the actual concentration of protein in the unknown samples it is necessary to draw the standard curve (concentration on the x-axis and on the absorbance y- axis) and interpolate the absorbance values of the unknowns. Draw graph and find protein concentration(mg/ml)
Transcribed Image Text:Table 1: The result for test tube 1 to 8 using of BSA stock solution. Tube BSA Cone H;O (ml) BSA (ml) Biuret ABS mg/ml reagent (ml) 1. 1.0 4. 0. 2. 0.9 0.1 4. 0.003 3. 2. 0.8 0.2 4. 0.012 4. 3. 0.7 0.3 4. 0.021 5. 4. 0.6 0.4 4. 0.028 6. 0.5 0.5 4. 0.039 7. 6. 0.4 4. 0.042 8. 7. 0.3 0.7 4. 0.052 Table 2: The result for test tube 9 to 12 using of Unknown solution Protein Tube Uknown (ml) H;0 (ml) Biuret reagent ABS concentration (mg/ml) (ml) 9. 4. 0.036 10. 4. 0.035 11. 0.5 0.5 4. 0.017 12. 0.5 0.5 4 0.016 In order to determine the actual concentration of protein in the unknown samples it is necessary to draw the standard curve (concentration on the x-axis and on the absorbance y- axis) and interpolate the absorbance values of the unknowns. Draw graph and find protein concentration(mg/ml)
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