Table 1. Sample Set A (PNPP concentration = 0.05mM) Final [Pi] Vol of 1mM P (μM) (HL) [PNPP] (mM) Vol of 0.5mM PNPP Vol of 0.2M Tris-HCI (μL) (μL) Vol of enzyme Total Vol (μL) (μL) 0 0.05 100 1500 3.33 0.05 100 1500 20 0.05 100 1500 50 0.05 100 1500 100 0.05 100 1500
Table 1. Sample Set A (PNPP concentration = 0.05mM) Final [Pi] Vol of 1mM P (μM) (HL) [PNPP] (mM) Vol of 0.5mM PNPP Vol of 0.2M Tris-HCI (μL) (μL) Vol of enzyme Total Vol (μL) (μL) 0 0.05 100 1500 3.33 0.05 100 1500 20 0.05 100 1500 50 0.05 100 1500 100 0.05 100 1500
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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S.s.s.s.s.s.g.g.g.f
![Table 1. Sample Set A (PNPP concentration = 0.05mM)
Final [Pi] Vol of 1mM P
(μM)
(HL)
[PNPP]
(mM)
Vol of 0.5mM PNPP Vol of 0.2M Tris-HCI
(μL)
(μL)
Vol of enzyme Total Vol
(μL)
(μL)
0
0.05
100
1500
3.33
0.05
100
1500
20
0.05
100
1500
50
0.05
100
1500
100
0.05
100
1500](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F67d3c839-d700-4710-8e8b-af5b5d99c8a3%2F5197be14-302b-421a-a442-8665645cd94f%2F1vsoc9o_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Table 1. Sample Set A (PNPP concentration = 0.05mM)
Final [Pi] Vol of 1mM P
(μM)
(HL)
[PNPP]
(mM)
Vol of 0.5mM PNPP Vol of 0.2M Tris-HCI
(μL)
(μL)
Vol of enzyme Total Vol
(μL)
(μL)
0
0.05
100
1500
3.33
0.05
100
1500
20
0.05
100
1500
50
0.05
100
1500
100
0.05
100
1500
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