A TLC is developed using a mixture of acetone/methanol (both polar solvents) as mobile phase. The solvent front runs 10 cm. Three spots A, B, and C are spotted with the following distances: A: 3.5 cm B: 8 cm C: 5 cm Which spot is the most polar?
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- 1. Answer true or false for each statement: a) A TLC analysis is sufficient to characterize the purity of a compound. b) Carboxylic acids containing six or more carbon atoms per molecule are more soluble in2.5 M sodium hydroxide than in diethyl ether. c) Phenols containing six or more carbon atoms per molecule are more soluble in 2.5 M sodium hydroxidethan in diethyl ether. d) Benzoic acid and p-nitroaniline form water-soluble salts, whereas naphthalene does not. e) TLC analysis is the method of choice to determine the type of solid phase and eluent system that is required for a column chromatography separation. f) Mineral oil is an inappropriate heating fluid for determining the boiling points of samples that exceed 200°C (760 torr)? g) A heating bath containing mineral oil should not be used to determine the melting points of solids meltingabove 200 °CA TLC of spinach leaves experiment is performed. The solvent front directly above the spot traveled 12.45 mm. The spot traveled 6.28 mm. What is the Rfvalue for the green pigment of the spot? Select one: 6.17 1.98 0.50 O 6.28please answer g
- hi, i need help on this one. It was rejected but i really need help on it! Thank youplease answer bPlant pigments in spinach were separated with TLC with cellulose as thestationary phase and 80/20 diethyl ether/acetone mixture as themobile phase. If needed, the solvent structures in the lab Appendix.1. Determine the Rf values of each plant component on this TLC plate.2. Arrange the plant components from most polar to most nonpolar. Please answer both 1 and 2 (picture below is the same question attached )
- Draw a GC chromatogram for a 50:50 and 25:75 mixtures of hexane (bp 68°C) and toluene (bp 111°C).5. Properties and IMF: Chlorophyll a and Chlorophyll b are almost identical in structure, but they separated during TLC. Report the RFs you recorded for both molecules. What functional group(s) are different between them? Then explain these RF values by comparing the IMF present in each molecule to those present in the mobile or stationary phase and how this shaped their affinities for each phase. Use the terms RF, IMF, polar, nonpolar, affinity, mobile phase and stationary phase.Polarities of analyte functional group increase in the order of hydrocarbon < ethers < esters < ketones < aldehydes < amides < amines < alcohols Arrange the order of elution [from shortest to longest retention time] for the following six compounds from an HPLC C18 column. C2H5OH CH3COCH3 CH3COOH CH3COOC2H5 C2H50C2H5 (СН3)2NH O A. C2H5OH < CH3COOH < (CH3)2NH < CH3COCH3 < CH3COOC2H55. A mixture was analyzed using TLC and it was doubted to contain three components: R, T, and Y. A non polar solvent was used with a polar stationary phase. Answer the following questions: cm 9. solvent front origin line R T Yrple Y mpte before lofter Which of the doubted components (R, T or Y) are present in the sample? Are there other components in the sample? What are the Rr values ofR and T?2) Consider the following compounds. Which rankings list these compounds in terms of Rf from lowest to highest using a silica gel thin layer chromatography plate and 1:1 Ethyl Acetate:Hexanes as the developing solvent. (e. g. AIf the stationary phase and the compound being tested are both polar, will the retention factor (Rf) likely be smaller or larger than if a nonpolar compound was tested? Why? 1) The retention factor will likely be smaller as it has a smaller interaction with the stationary phase O 2) The retention factor will likely be greater as it has a smaller interaction with the stationary phase O3) The retention factor will likely be smaller as it has a greater interaction with the stationary phase 4) The retention factor will likely be greater as it has a greater interaction with the stationary phaseRecommended textbooks for youChemistryChemistryISBN:9781305957404Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCostePublisher:Cengage LearningChemistryChemistryISBN:9781259911156Author:Raymond Chang Dr., Jason Overby ProfessorPublisher:McGraw-Hill EducationPrinciples of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage LearningOrganic ChemistryChemistryISBN:9780078021558Author:Janice Gorzynski Smith Dr.Publisher:McGraw-Hill EducationChemistry: Principles and ReactionsChemistryISBN:9781305079373Author:William L. Masterton, Cecile N. HurleyPublisher:Cengage LearningElementary Principles of Chemical Processes, Bind…ChemistryISBN:9781118431221Author:Richard M. Felder, Ronald W. Rousseau, Lisa G. BullardPublisher:WILEYChemistryChemistryISBN:9781305957404Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCostePublisher:Cengage LearningChemistryChemistryISBN:9781259911156Author:Raymond Chang Dr., Jason Overby ProfessorPublisher:McGraw-Hill EducationPrinciples of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage LearningOrganic ChemistryChemistryISBN:9780078021558Author:Janice Gorzynski Smith Dr.Publisher:McGraw-Hill EducationChemistry: Principles and ReactionsChemistryISBN:9781305079373Author:William L. Masterton, Cecile N. HurleyPublisher:Cengage LearningElementary Principles of Chemical Processes, Bind…ChemistryISBN:9781118431221Author:Richard M. Felder, Ronald W. Rousseau, Lisa G. BullardPublisher:WILEY