A TLC is developed using a mixture of acetone/methanol (both polar solvents) as mobile phase. The solvent front runs 10 cm. Three spots A, B, and C are spotted with the following distances: A: 3.5 cm B: 8 cm C: 5 cm Which spot is the most polar?

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A TLC is developed using a mixture of acetone/methanol (both polar solvents) as the mobile phase. The solvent front runs 10 cm. Three spots A, B, and C are spotted with the following distances:

- A: 3.5 cm
- B: 8 cm
- C: 5 cm

**Question:** Which spot is the most polar?

- ○ A
- ○ B
- ○ C
- ○ Both B and C

**Explanation:** In thin-layer chromatography (TLC), the most polar compounds tend to travel the least distance on the plate, since they interact more strongly with the polar stationary phase. Therefore, the spot that moves the least distance from the origin is generally considered the most polar. Based on the distances provided, spot A is the most polar, as it traveled the shortest distance (3.5 cm).
Transcribed Image Text:A TLC is developed using a mixture of acetone/methanol (both polar solvents) as the mobile phase. The solvent front runs 10 cm. Three spots A, B, and C are spotted with the following distances: - A: 3.5 cm - B: 8 cm - C: 5 cm **Question:** Which spot is the most polar? - ○ A - ○ B - ○ C - ○ Both B and C **Explanation:** In thin-layer chromatography (TLC), the most polar compounds tend to travel the least distance on the plate, since they interact more strongly with the polar stationary phase. Therefore, the spot that moves the least distance from the origin is generally considered the most polar. Based on the distances provided, spot A is the most polar, as it traveled the shortest distance (3.5 cm).
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