A solution of DNA of unknown concentration (termed "[unknown] DNA solution") gave an absorbance value of 0.39 at 260 nm. The goal here is to determine the DNA concentration of this [unknown] DNA solution. To calculate this, you are given the following additional information: A DNA solution whose concentration is 50 ug/ml has an absorbance value of 1.0 at 260 nm. Using this information and knowing that A1/C1 = A2/C2 i. Calculate the DNA concentration (ug/mL) in the DNA solution of [unknown] ii. Once you have determined the DNA concentration of [unknown] DNA solution, calculate how many ug of DNA would be present in 25 μl of this DNA solution iii. iv. If you dilute the "[unknown] DNA solution" 10-fold, i.e. 1/10, what will the absorbance value (at 260 nm) of the diluted solution be? What will the new concentration of DNA of the diluted solution be?

A solution of DNA of unknown concentration (termed "[unknown] DNA solution") gave an absorbance value of 0.39 at 260 nm. The goal here is to determine the DNA concentration of this [unknown] DNA solution. To calculate this, you are given the following additional information: A DNA solution whose concentration is 50 ug/ml has an absorbance value of 1.0 at 260 nm. Using this information and knowing that A1/C1 = A2/C2 i. Calculate the DNA concentration (ug/mL) in the DNA solution of [unknown] ii. Once you have determined the DNA concentration of [unknown] DNA solution, calculate how many ug of DNA would be present in 25 μl of this DNA solution iii. iv. If you dilute the "[unknown] DNA solution" 10-fold, i.e. 1/10, what will the absorbance value (at 260 nm) of the diluted solution be? What will the new concentration of DNA of the diluted solution be?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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