viii) You have the following stock solutions available: 2.5M Tris, 0.5M Glycine and 10% SDS, how would you prepare a 1 Litre solution of 25mM Tris, 190mM Glycine and 0.1% SDS for use in a protein separation experiment.
Q: A typical amino acid with one amino group and one carboxylicacid group, such as serine, can exist in…
A: At lower pH that means in acidic conditions the carboxylate ion takes up proton and exists as…
Q: A typical amino acid with one amino group and one carboxylic acid group, such as serine can exist in…
A: At lower pH that means in acidic conditions the carboxylate ion takes up proton and exists as…
Q: 2) A student standardizes Iz using 0.250g of pure ascorbic acid. It takes 35.50ml to reach a starch…
A: Iodine is standardized using ascorbic acid as it is available in high pure form and is a weighable…
Q: Fig. 1 shows the possible effect of pH on recovery and impurity removal at the selection of a…
A: The Y-axis of the graph represents the percent recovery of the impurity, and the X-axis of the graph…
Q: The first step in the procedure asks you to prepare five dilutions of the protein standard (BSA) in…
A: During the dilution process, solvent is added to the solution and hence changing the concentration…
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Q: A mixture of seven amino acids (glycine, glutamate, leucine, lysine, alanine, isoleucine, and…
A: Chromatography is the technique that is used to separate the amino acids based on the priority and…
Q: The UV-vis absorption spectrum of the peptide RYYFE collected in a 1 cm pathlength cuvette exhibits…
A: the optical path length and the molar concentration of the solution. i.e.…
Q: Rank the amino acids used in the experiment in Part II in the order of increasing solubility in…
A: The solubility of amino acids, generally, depending upon the nature of the side chain and they are…
Q: A sample containing histidine (pKas 1.8, 6.0, 9.3), lysine (pKas 2.2, 9.1, 10.5), and leucine (pKas…
A: Because a protein's net charge is pH-dependent, the sample may be eluted from IEX medium by varying…
Q: A purified protein is in a HEPES (N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)) buffer at…
A: Given: concentration of NaCl = 600 mM Since, 1 mL of sample, 600 mM NaCl and HEPES buffer is…
Q: Consider an amino acid with the following pKa values: pKa1 = 2.71 and pK, = 9.31 At what pH value in…
A: Amino acids are amphoteric in nature due to the presence their ionizable α-amino and α-carboxylic…
Q: At pH 5.0? Q2) (a) Draw the structure of the predominant form (principal species) of…
A:
Q: A chromatogram showing the results for an amino acid test show a solvent line of 7.5cm. If the amino…
A:
Q: Tyrosine gave a positive reaction to these tests except l. Hopkins-Cole |I. Biuret I.Sakaguchi IV.…
A: Hopkins Cole test - to detect tryptophan Biuret test - for present of peptide bond and…
Q: What would be a suitable blank for an assay where the samples contain protein in a solution with dye…
A: A question based on absorption that is to be accomplished.
Q: Use the following chromatogram of standard amino acids: 24 16 19 21 12 15 6,6 9 10 23 13 14 17 18 10…
A: In chromatography, a higher retention time is linked to a higher affinity for the stationary phase…
Q: The electrophoresis of a sample containing 2-amino-4-methylpentanoic acid. (pKa of a-COOH = 2.2, pKa…
A:
Q: A student performed a chromatography experiment to determine the Rf value of an unknown amino acid.…
A: The Rf value ( retention factor) of a compound is defined as the ratio of distance travelled by the…
Q: Find the isoelectric and zwitter ion of lysine
A: isoelectric point means the characteristic pH at which net effective charge of an amino acid is…
Q: An antibody X binds to an antigen Y. When the antigen concentration is 3.0 x 10 M, 0 is 0.50. What…
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Q: Which most accurately describes what the diagram below represent? Reflected light Incident light…
A: It's a Schematic diagram of an SPR based genosensor. The incident polarized light is coupled by a…
Q: they have a binding site for X with a Kd (dissociation constant) of 10⁻⁵and 10⁻⁸ M, respectively.…
A: For comparison association constant of each is determined. The one having highest value of…
Q: A protein with a pI of 7.5 will be purified using ion-exchange chromatography. Which ion exchange…
A: b) A resin with sulfonic acid group is the correct option.
Q: In Kjeldahi analysis, a flour sample weighing 0.9857 was digested in concentrated H,So, for 45 min.…
A: Given the volume of 0.1011 N H2SO4 treated with NH3 extracted from the sample = 50.00 mL * (1L/1000…
Q: 5.00 mL sample of filtered orange juice was treated with 50.00 mL of 0.01019 M I3– to oxidize…
A: Idometric titration:
Q: which of the following chromatographic methods is best used for separating amino acids?
A: Chromatography is the technique that is used for the separation of different components from the…
Q: For the determination of Pb in blood, a 5.00 mL sample was treated with trichloroacetic acid to…
A: The given data is as follows. A1 = 0.396 (first point) A2 = 0.599 (second point) C1 =…
Q: While purifying a new protein using a dextran Sephadex, the consistency of the column material…
A: As per the bartleby expert guidelines, I am allowed to answer one question at a time . Please…
Q: 3. Isoelectric focusing is a separation technique that takes advantage of the differences in…
A: Isoelectric focusing: It separates the different amino acids based on their differences in their pI…
Q: QUESTION 1 You obtained the following raw data when setting up a Bluret standard curve BSA (mgim)…
A: Calibration curve between [BSA] versus Absorbance Considering the linear least regression equation,…
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A: Since you have posted a question with multiple sub-parts, we will solve first three subparts for…
Q: If prior to the analysis, the protein X needs to be solubilised in 250 mM acetate buffer, pH 4.0 to…
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Q: Question attached
A: Paper chromatography is a qualitative method in order to detect the presence of colored components.…
Q: Your HRP sample had a concentration of 2.0 mg/mL and you added 10 uL to a tube containing 10 uL of…
A: Concentration of HRP sample = 2.0 mg/mL
Q: Cuivan Coreeak base C₂H₂0 Nuo ₂. a 2. kb = 3.98 x 10-11 trund the PM of a Solution prefered by…
A: Here we are required to find the pH of weak base solution.
Q: 65mL of a 0.2M solution of the tetrapeptide Arg-His-Ile-Glu is adjusted to pH=7.8. The solution is…
A: Here, we are titrating a tetrapeptide Arg-His-Ile-Glu against HCl.
Q: How does the shape of a titration curve confirm the fact that the pH region of greatest buffering…
A: As there are three question are present I answered only one. you can repost your remaining two…
Q: 1) Compare between three steps for Kjeldahl method for determination of protein?I
A: INTRODUCTION: Kjeldahl method is defined as the method which is used for quantitative determination…
Q: A 5.00 mL sample of filtered orange juice was treated with 50.00 mL of 0.01019 M I3– to oxidize…
A:
Q: what are the advantages of mediator-based amperometric enzyme sensors for glucose compred to…
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Q: A 5.00 ml sample of filtered orange juice was treated with 50.00 mL of 0.01019 M I3 to oxidize…
A: To calculate concentration of ascorbic acid , we need to calculate the moles of ascorbic acid . For…
Q: P UTF-8 Chapter 12 Solutions-20 x+…
A: Given-> Initial Volume = 2.0 L Initial molarity = 6.0 M Final volume = 6.0 L On dilution moles…
Q: A purified protein is in a HEPES (N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)) buffer at…
A: Initial concentration of NaCl solution, Minitial = 600 mM Initial volume taken, Vinitial = 1 mL…
Q: Name four factors (bonds or other forces) that contribute to stabilizing the native structure of a…
A:
Q: 13.) A mixture containing 50% mole of A is distilled at a temperature T, what is the distillate to…
A: In relation to molar concentration, mole fraction is temperature independent Here variation of…
Q: The pKa values for the α-carboxylic acid and the α-amino acid groups is cysteine are 1.092 and…
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Q: You would like to calculate the concentration of a protein in a solution measured in a 96 well…
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- The ethyl acetate concentration in an alcoholic solution was determined by diluting a 10.00-mL sample to 100.00 mL. A 20.00-mL portion of the diluted solution was refluxed with 40.00 mL of 0.04672 M KOH:CH3COOC2H5 + OH- → CH3COO- + C2H5OHAfter cooling, the excess OH2 was back-titrated with 3.41 mL of 0.05042 M H2SO4. Calculate theamount of ethyl acetate (88.11 g/mol) in the original sample in grams(5) How do you prepare 25mL of a mix solution of 2% (v/v) serum and 10mM EDTA in 1X PBS. You have been provided with: 0.5M EDTA stock solution 10X PBS Liquid serumThe separation and purification processes given below and the method used against them are given. Which or which of these pairings are correct? Brewing the tea - Extraction Purification of water from impurities-Chromatography Separating olive pomace from oil while olive oil is being produced - Decantation Obtaining oil fractions - Ordinary distillation Obtaining essential oils and using them in perfume making - Water-steam distillation A. I, IV, V B. I, II, III, IV, V C. I, II, V D. I, II, III, V E. I, II, III, IV
- A 10.00mL sample of alcoholic ethyl acetate was diluted to 100.00 mL. 20.00 mL was aliquoted and mixed with 40.00 mL of 0.04672 M KOH. The resulting mixture was heated for 2 hours. CH3COOC2H5 + OH- → CH3COO- + C2H5OH After cooling, the excess OH was back titrated with 3.41 mL of 0.05042 M H2SO4. Answer the following: Calculate the number of moles of OH- that reacted with ethyl acetate. Calculate the number of moles of ethyl acetate in the 20.00 mL solution. What is the mass of ethyl acetate (FW=88.11 g/mol) in the original 10.00 mL sample?Solute, A, has a distribution ratio between water and ether of 7.5 A 50 ml aqueous solution of 1M of compound A was extracted with two consecutive extraction of 25ml ether( 25 x2); calculate the percent of A remaining in the aqueous phase Select one: 21.1 % 1.6 % 4.4 % 11.7 % 1.38 %What is the molarity of an unknown HCl solution with a specific gravity of 1.10 and an assay percentage of 18.5 %? (atomic weight: H = 1.00794, Cl = 35.4527) PLS SHOW SOLUTION
- A K2SO4 solution is prepared by weighing out a certain amount of the pure solid reagent,dissolving it to 500.0 mL in a volumetric flask. An aliquot was taken from this solution.of 25.00 ml and diluted to 500.0 ml. An aliquot of 5.00 was taken from this new solution anddiluted to 50.00 ml. A chemical analysis of this new solution showed a 0.100%(w/v)in K+. Calculate the weight of the pure solid K2SO4 used for the preparation of the original solution.and its concentration in each volumetric flask. The answer is 2.23x10^2gcalculate the weight of Sodium carbonate normality 0.1 N and prepared -1 * ? with 100 ml distilled water, given that the molecular weight was 106 gm(DV 20 ml. of an aqueous solution of 2 mmol butyric acid is shaken with 10 mL ether. After the layers are separated, it was found that 0.5 mmol of butyric acid remains in the aqueous layer. Calculate "D" and "%E".
- Calculate the mass percent of the ascorbic acid in the same show full solution Situation: The pounded Vitamin C tablet was half into two below are the calculated data in the experiment. Each of the half are transfered in a 250ml erlen meyer flask and added 75ml of deionized water for the vitamin C to dissolve. Then it was prepared for titration where the burette was filled with 0.09794M standardize NaOH solution then a total of with 3 drops 2% phenolphthalein indicator was added to each erlen meyer flask l, swrill it and begin the titration. below are the following data gathered: Vitamins used: Nature Made vitamin C 500mg tablet Trial 1 Mass: 0.3108g of Vitamin C tablet Initial volume: 1.6mL Final volume: 15.4mL Trial 2 Mass: 0.3349g Initial volume: 15.4 mL Final volume: 30.2 mLshould i use fractional or simple distillation for the following: separation of 1-butanol (Tb = 116 oC) from ethanol (Tb = 78 oC).? Only typed solution2. You need to make 500mL of 7H9(+) media with 1X ADN and 30ug/ml. of the antibiotic kanamycin. Your stock solutions are 10X ADN and 30mg/mL of kanamycin. The solvent is distilled water. How much of cach component will you need to make the desired final solution?
