You obtained the following raw data when setting up a Bradford standard curve:BSA (mg/ml)Absorbancy595nm00.15510.28720.42030.52340.65150.75660.87870.91880.9549100.9820.990After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method andan absorbancy of 0.566 was obtained.Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample inmg/ml (up to 3 decimal places).

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Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Table 2: Absorbance Values of Standards and Unknowns. Sample Blank Standard Solution 1 Standard Solution 2 Standard Solution 3 Standard Solution 4 Standard Solution 5 Water Sample 1 Water Sample 2 Phosphate Concentration (in ppm) Y17 0 ppm 0.02 ppm 0.04 ppm 0.08 ppm 0.16 ppm 0.32 ppm Freeman Lake 0.001 0.325 0.292 0.413 0.315 0.039 0.054 0.049 Absorbance at 2= 690 nm Calculations: 1. Construct a phosphate standard curve in Excel by plotting concentration (in ppm) on your x-axis and Absorbance (unitless) on your y-axis for your known solutions. Label the axes on the graph and provide the curve with a title. Use a linear trendline to generate a best fit line to your data. Label the graph with the equation and the R" value. Insert your labeled graph in the space below. X
In a Bradford assay, 11 µl of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 µg/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 13 4 6 conc of BSA 12.00 24.00 36.00 48.00 60.00 (pg/mL) Absorbance at 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.
You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.523 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
1. Data & Results 4.0 mglmL Concentration of stock solution (include units): Table 1-Beer's Law Plot Data V Volume of standard solution dispensed (mL) M, Diluted concentration (include units) Sample Absorbance A 2.00 mL 0.084 4.00 mL 0.173 l6.00 mL 0.259 8.00 mL 0.340 E 10.00 mL 0.418 Equation of trendline from Beer's Law plot: whats the diluted concentration and equation of trendline from the beers law plot once created?
The absorbance of an unknown dye solution is measured to be 1.06 by a spectrometer. The calibration plot of the spectrometer is provided below. What is the molar concentration of the unknown sample (in mole L)? Absorbance vs. Molarity 18 y3 67024x-0.0343 16 14 12 1 0.8 0.6 0.4 0.2 01 0.2 0.000005 00001 0000015 Concentration of solutions, mol/L 0.00002 0.000025 000003 0 163e-5 O 2.35e-6 0 163 0 235 Absorbance
The protein content of the unknown samples were determined using BSA as standard. The absorbance values of the standard solutions and samples are as follows. BSA concentration (mg/mL) Absorbance 0.000 20 0.110 40 0.192 60 0.248 80 0.325 100 0.400 120 0.460 a. What is the equation of the line? b. Determine the protein concentration of samples in ug/mL. Sample dilutions 1 in 14 A540 0.150 1 in 5 1 in 8 0.380 0.235
auDUIUance UI U.I JU ai 4JJ IIII III 1. UU CIII cuvclic. Calculate the molar absorptivity of the pigment at 453 nm? Q5) A student is tasked to determine the number of moles of H2SO4 (aq) in a 200 ml of an unknown solution. The student prepares a series of solutions with known concentrations of H2SO4 (ag) and measures the absorbance of each at an appropriate wavelength. These data are used to construct the calibration plot shown below. 1.00 0.80- 0.60 - 0.40- 0.20- 0.00- 3 x 10-4 6 x 10-4 Concentration (mol/L) If the absorbance of the unknown solution is 0.70, how many moles of H2SO4 (aq) does the 200 ml sample contain? Absorbance
You obtained the following raw data when setting up a Biuret standard curve: Absorbancy 540nm BSA (mg/ml) 0.158 1 0.210 2 0.260 3 0.305 4 0.360 0.410 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.279 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
Plot a professional quality standard curve (absorbance vs. protein amount in µg) with an appropriate title and caption underneath the plot from the data give above. The caption should include the best-fit data.
The spectroscopic data in the table is generated with five solutions of known concentration. Concentration (M) 0.0133 m= 0.0266 0.0532 0.106 0.213 Absorbance 0.1271 What is the intercept of the linear regression line? 0.08531 0.5388 1.069 Use a spreadsheet program, such as Microsoft Excel, to graph the data points and determine the equation of the best-fit line. 1.954 What is the slope of the linear regression line formed by these points? M-1
10 Wavelength, 1. (nm) E (Red, µ.Mcm- ɛ(Yellow, µMr'cm¬1) Absorbance 500 0.0369 0.000549 0.405 430 0.00506 0.0167 0.474 An orange colored solution is prepared by mixing a red dye solution with a yellow dye solution and the absorbance was measured at the analytical wavelengths of 430 nm and 500 nm. The table provides the molar absorptivity for the red and yellow dye at each analytical wavelength. Calculate the concentration (in u.M) of the red and yellow dye in the mixture. [red] = [yellow] =
6. Blue Blue dye stock solution 0.293 M Absorbance at 630 nm 0.00265 Calibration curve y = 0.0833x A solution is prepared by diluting 2.79 mL of the blue dye stock solution to 25.00 mL. The measured absorbance for the prepared solution is listed in the data table. (a) What is the theoretical molar concentration? [Blue]theoretical x 10 |M (b) What is the experimental molar concentration? [Blue]experimental x 10 M (c) What is the percent error? Percent error (blue) = %

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