You obtained the following raw data when setting up a Bradford standard curve:   BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990   After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.523 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).

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A: Given dataFe(II)SO4 , Concentration,mMAbsorbance 0010.18820.38830.55840.749Now plot the data on x-y…

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A: 1.)  We would use formula of Absorbance.        Note : As you have uploaded two questions, I'll…

Q: Q5) A student is tasked to determine the number of moles of H2SO4 (aq) in a 200 ml of an unknown…

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Q: Sarah wasn't sure about Ben's results, so she repeated the experiment and found the best-fit line…

A: The molarity and volumes of reactants initially taken are: 5.00 mL of 0.002 M Fe(NO3)3 or Fe3+(aq)…

Q: Find (M) for [FeSCN^2+] using the following info: 0.200 M (Iron (III) nitrate) Fe(NO3)3 (mL) = 17.98…

A: The volume of 0.200 M Fe(NO3)3 solution is 17.98 mL. The volume of 0.200 M KSCN solution is 1.97 mL.

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Q: Fe+3 (aq) + SCN – (aq) FeSCN +2 (aq)   She mixes 4.00 mL of 2.00 mM SCN– and 5.00 mL of 2.00 mM Fe+3…

A: Given: ℓ = 1.0 cm ε =2867 M

Q: Unknown Sample Calculations Sample y (absorbance) = m x (concentration) + b 1 0.200 = 0.1107…

A: A generalized formula helpful in calculating absorbance for any specific solution with known…

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Q: for density is M/\ Calculate the molarity of solution prepared by weighing 6.34 g of NaOH using a…

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Q: A solution contains 0.10 M Sr(NO3)₂ and 0.20 M Bi(NO3)₂. When solid Na AsO is added to the solution…

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Q: A student used 500mg of tabletop sweetener to make a total of 5ml of solution with biuret reagent.…

A: The student prepared a solution containing 500 mg of tabletop sweetener in a total volume of 5 mL.…

Q: ou obtained the following raw data when setting up a Biuret standard curve:   BSA (mg/ml)…

A:

Q: The nitrite in a series of standard solutions (mg/L, n = 5) are converted to azo dye and the slope…

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Q: What is the structure of A and B? M. A= A= A= A= Un.. Himos וווווו OH Br Br Br PB13 Pyridine Br B=…

A: Given reaction is an example of nucleophilic substitution reaction via the SN2  type reaction.

Q: A CaCO3 solution that will be used to standardize EDTA was prepared by dissolving 3.1251 g of solid…

A: Given data; Mass of solid CaCO3 is 3.1251 g The volume of dilute HCl  for prepare CaCO3 solution is…

Q: The concentration of Ba(1O3)2 in H2O at 35°C (Ksp = 3.00 x 10-9) Ba(103)2 (s) %3D Ba2+ + 2103 O 5.23…

A:

Q: The answer is 0.372 ug/mL

A: Please see the attached image for the solution.  If there are queries or if the image is not…

Q: A toluene solution (1.50 mg / L) shows an absorbance of 0.30 in a cuvette with a 0.025 mm optical…

A: Given that  Absorbance of Toluene solution is = 0.30 Cuvette path length = 0.025 mm Concentration=…

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A: The given expression for the linear equation for the spectrometr calibration is ; Y = 2156.5 X +…

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Q: I need to know: 1. moles FeNCS2+ at equilibrium (10mL) (mol) 2. moles Fe3+, reacted (mol) 3.…

A: Solution: Here first we write the given reaction for the reference.  Fe3+ (aq) + SCN- (aq) ====>…

Q: Tooth enamel is composed of the mineral hydroxyapatite, Ca5(PO4)3OH which has a Ksp of 6.8 x 10-37.…

A: Step 1: Step 2: Step 3: Step 4:

Q: Ben ran through the experiment and found the best-fit line listed below for his standard calibration…

A: Given , absorbance of solution = 0.432  Volume of 0.002 M iron(III) nitrate = 5.00 mL Volume of…

Q: (a) A supplement tablet containing about 300.00 mg of calcium ion was dissolved, filtered, and…

A: [EDTA] = 0.0100 M Volume of titration volume of EDTA =…

Q: if its absorbance is 0.900, find the molar absorptivity of a solution containing 3.0 x 10-3 g/L…

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Q: ± 0.00098 18 Bromocresol green 0.08641 ± 0.00113 29 Is the difference between indicators

A: 95 % confidence interval for a population mean can be represented as

Q: A series of caffeine standards were prepared and scanned with an IR spectrophotometer yielding the…

A: The above graph to determine the percentage of caffeine in aqueous solution of sample and to prepare…

Q: The standard curve was made by spectrophotographic analysis of equilibrated iron(III) thiocyanate…

A: Here  we are required to find the Fe3+ concentration of the unknown solution.

Q: .0 mL of an unknown FeCl3 solution is diluted to a total volume of 230.0 mL. The diluted  FeCl3…

A: Given that: Initial volume of FeCl3 = 45.0 mLFinal volume of FeCl3 = 230.0 mLAbsorbance = 0.223Molar…

Q: In a 250.0 mL beaker, 10.0 mL 0.200 M H3PO4, 20.0 mL 0.400 M Na3PO4, 10.0 mL of 0.200 M NaOH and…

A:

Q: The preparations were randomly drawn from the same population. a. Find the mean and standard…

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Q: If the absorbance for the solution was 0.698, calculate the equilibrium concentration of iron(III)…

A: It is  based on the Lambert  Beer Law, Here we are required to find the equilibrium concentration of…

Q: The concentration of Ba(103)2 in H2O at 35°C (Ksp = 3.00 x 10- %3D Ba(103)2 (s) - Ba²* + 2103 5.23 x…

A: Ksp = 3.00 × 10-9  Concentration of Ba (IO3)2 = ?

Q: What is limit of detection? What is standard deviation?

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Q: The dota shown below is calibration data obtained from the determination of a pesticide in soil…

A: 1.  Concentration = 3.6

Q: A graphite furnace atomic absorption spectrophotometer (AAS) was used to determine the arsenic (As)…

A: Given data Concentration Absorbance 2 0.17 4 0.28 6 0.48 8 0.53 10 0.72

Q: A researcher prepared mixed solutions at different concentrations and measured the absorbances with…

A: A question based on Beer-Lambert law, which is to be accomplished.

Q: You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) 0 1 2 3 4 5…

A: