In a Bradford assay, 11 µL of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 ug/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 3 6 conc of BSA (rg/mL) 12.00 24.00 36.00 48.00 60.00 Absorbance lat 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.

In a Bradford assay, 11 µL of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 ug/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 3 6 conc of BSA (rg/mL) 12.00 24.00 36.00 48.00 60.00 Absorbance lat 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

Suppose that a solution has an absorbance of 0.250 at a wavelength of 450 nm. If the concentration of the solution is 22 μM, what is the value of the molar absorptivity? The data were taken with a standard 1-cm cuvette. molar absorptivity in (L cm^−1 mol^-1):
SPECTROPHOTOMETRY Two groups in a BIO 120 lab extracted a blue-violet pigment from mayana plant which was to be used as a natural dye for Philippine textiles. To determine the concentration of the pigment isolated, each group made a standard curve using commercially available indigo dye in dimethylsulfoxide (DMSO). The following data were obtained by the two groups: Table 4. Absorbance readings of standard solutions of varying concentrations of indigo dye in DMSO. Pure DMSO 0.115 Standard solutions (ppm) Group 1 Data Group 2 Data 0.2 0.398 0.224 0.4 0.563 0.437 0.6 0.732 0.603 0.8 0.833 0.811 1.0 1.008 0.966 1. Show the constructed standard curves of the two groups with proper figure titles. 2. Which of the two groups generated a better standard curve? Justify your answer by providing the R? value for each data set. 3. The extracted pigment has an absorbance of 0.983. When a 0.2 mL-aliquot of the extract was diluted to 1.0 mL, the absorbance of the diluted solution was found to be…
The students conducted the assay for LDH activity using the two serum samples. Each cuvette (path length 1 cm) contained 3ml of a suitable assay buffer (including pyruvate as substrate). 20 microlitres of either serum was added to the cuvette and the absorbance values immediately recorded at the optimum wavelength for a period of 5 minutes (absorbance readings taken every 30 seconds). Protein concentration of serum sample (mg/ml) Change in absorbance at optimum wavelength per minute Control serum (C) 8 -0.04 Diseased serum (D) 7.8 -0.6 1c. Using the molar absorption coefficient of NADH as 6220 M-1 cm-1, and by application of the Beer-Lambert law, estimate the enzyme activity in the two samples (C and D). Express activity as moles per second. 1d. Estimate the specific activity of the two samples (moles per second per microgram).
A liquid sample was analyzed for Fe³+. A plot of absorbance (y-axis) vs. concentration (ppm; x-axis) of the external standards produced a calibration line with an equation y = 0.2256x -0.0903 (R² = 0.9973). The absorbance of the sample was 1.25. Determine the amount of Fe³+ in the sample (in ppm).
2) In a laboratory study, a calibration was performed using NH4C1 to analyze ammonium spectrophotometrically. The results of the study are given below. According to this, when the 5.0 mL sample containing ammonium is completed to 50 mL with pure water, find the amount of NH4 in the sample whose absorbance was found as 0.12 according to the table. (N: 14 g / mol, H: 1 g / mol, Cl: 35.5 g / mol). Concentration (mg/L) Absorbance 0.01 5 0.05 7.5 0.1 10 0.15 12 0.18
A young researcher set out to develop a method for quantifying the polycyclic aromatic hydrocarbon (PAH) content in a contaminated soil sample using open tubular gas chromatography. During the initial stages of method development, the researcher was unable to separate the early eluting compounds, anthracene and naphthalene, with baseline resolution. Select the possible modifications the researcher could make to improve the resolution. use a column with a smaller inner diameter decrease the column length increase the stationary phase thickness increase the temperature choose a different stationary phase
What is the best Chromatography instrument for analysis of Soluble Protein molecules CG-FID GC-MS LC-MS IC-MS O IC-EC
A purple solution is prepared by mixing 5.00 mL of the blue dye solution with 6.25 mL of red dye solution. The calibration curve for each color is provided. Measurement Result Measurement Blue dye stock solution (M) Result 0.1944 M Red dye stock solution (M) 0.2015 M Absorbance at 630 nm 0.00697 0.00193 Absorbance at 500 nm Calibration curve Calibration curve y=0.0682x y=0.0170x Part: 0 / 4 Part 1 of 4 Determine the theoretical molar concentration of each dye in the mixture. Round your answer to 3 significant digits. [Blue theoretical = M D [Red theoretical M X Part: 1/4 Part 2 of 4 Using the absorbance and calibration curve for each dye, calculate the experimental molar concentration of each dye in the mixture. Round your answer to 3 significant digits. [Blue] M Submit A Continue © 2022 McGraw I LLC. All Rights Reserved. Terms of Use | Privacy Center 12 W 10 O
How to solve the following?
A student developed a new dye called Dye C. It was analyzed against other dyes using paper chromatography. A solution of 1:1 ethyl acetate:hexane was used as the eluent. The resulting chromatogram is shown in the figure. Determine the retention factor (Rf) values of each spot on dye C. (use 2 decimal places) What other dye/s are present in dye C and what is the dye with least number of pigments?