In a Bradford assay, 11 µL of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 ug/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 3 6 conc of BSA (rg/mL) 12.00 24.00 36.00 48.00 60.00 Absorbance lat 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.

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In a Bradford assay, 11 µl of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein
concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution.
The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose
concentration is 200 µg/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table
below:
Standard soln #
1
2
13
4
6
conc of BSA
12.00
24.00
36.00
48.00
60.00
(pg/mL)
Absorbance
at 595 nm
0.000
0.156
0.249
0.328
0.411
0.528
zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.
Transcribed Image Text:In a Bradford assay, 11 µl of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 µg/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 13 4 6 conc of BSA 12.00 24.00 36.00 48.00 60.00 (pg/mL) Absorbance at 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.
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