A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was found to be 200.8 s with a baseline peak width, we, of 16.1 s. The retention time for aspartame, ta, was 258.7 s with a baseline peak width, wa, of 20.6 s. The retention time for the unretained solvent methanol was 44.2 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column. H = 88.2 Calculate the resolution, R, for this separation using the widths of the peaks. R = 3.16 Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5. μm

A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was found to be 200.8 s with a baseline peak width, we, of 16.1 s. The retention time for aspartame, ta, was 258.7 s with a baseline peak width, wa, of 20.6 s. The retention time for the unretained solvent methanol was 44.2 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column. H = 88.2 Calculate the resolution, R, for this separation using the widths of the peaks. R = 3.16 Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5. μm

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter27: Gas Chromatography

Section: Chapter Questions

Problem 27.19QAP

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Question

A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was
found to be 200.8 s with a baseline peak width, we, of 16.1 s. The retention time for aspartame, ta, was 258.7 s with a baseline
peak width, wa, of 20.6 s. The retention time for the unretained solvent methanol was 44.2 s.
Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column.
H =
Calculate the resolution, R, for this separation using the widths of the peaks.
R =
88.2
R₂5N =
3.16
Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5.
3.533
Incorrect
μm

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