Here is the protocol for a UV-Vis spectrophotometer to detect water and chlorine-carbon. 1.Dissolve the water and chlorine-carbon compounds in a solvent, such as water. 2.Prepare a standard solution of known concentration that is similar to the sample being measured. 3.Calibrate the spectrophotometer using the standard solution. 4.Measure the absorbance of the sample using the spectrophotometer. 5.Calculate the concentration of the compounds in the sample using the calibration curve obtained from the standard solution. How is the spectrophotometer calibrated with standard solutions? When is the blank solution placed in the spectrophotmeter?

Q: Following the experimental procedure described in the lab manual, a student combined 5.00 mL of…

A: Since you have posted a question with multiple sub-parts, we will solve first three sub-parts for…

Q: Question 3 Why is it necessary to run a blank before measuring the absorbance of a sample? O To…

A: Why is it necessary to run a blank before measuring the absorbance of a sample ? The reason is given…

Q: 3. Using the data at right: a. Calculate the absorbance of each sample. You will need to express the…

A: We need to calculate the absorbance for each standard concentration and then plot the absorbance vs…

Q: 3. If you wished to study the molecule below by measuring absorbance, what wavelength should you set…

A: In absorbance study analyte solution absorb the incidents wavelength intensity.

Q: 100 Unknown Liquid 75 70- 65 60 55 50 45 40 35 30 25 20 15 10 5 4000 3500 3000 2500 2000 1500 1000…

A: IR spectra is the spectra that uses the IR radiation to excite the molecule where the IR spectrum is…

Q: C. Spectrophotometry 1. Measure the absorbance of the standard solutions and construct a calibration…

A: A question based on calibration of curve that is to be accomplished.

Q: A takes polychromatic light and provides monochromatic light to pass on to the sample and then…

A: Monochromatic light : The light having single wavelength is called monochromatic light and light…

Q: For this experiment depicted in the picture, Interpret the most important absorbances in the IR…

A: A question based on IR spectrum, which is to be accomplished.

Q: 4.00 3.50 3.00 2.50 2.00 1.50 1.00 y = 19883x + 0.5405 R? = 0.8873 0.50 0.00 0.00E+00 5.00E-05…

A: This can be obtained by putting drop line

Q: Choose the best answer. Background correction for atomic spectroscopy is important, because: A. It…

A: In spectrometric analysis, Background Correction is one of the important processes. The non-specific…

Q: For the experiment this week, you'll use the relationship between FeSCN2* absorbance and…

A:

Q: Hi please answer all and below the answe place the URL from where you got the answers thank you

A: Hey, since there are multiple questions posted, we will answer first question. If you want any…

Q: QUESTION 4 4. The main difference between "in-time" and "in-space" spectrophotometer design is that…

A: The terms "in-time" and "in-space" spectrophotometer designs refer to different methods of acquiring…

Q: alculate the concentration of the unknown MnO4 sample, given the following standard addition…

A: We have to use Lambert-Beer's law A=€cl Here A-absorbance,  €= molar absorbtion coefficient C=…

Q: Do your graphs show the trends a)you expected based on what you know about absorbance and…

A: Absorbance of the substance is the amount of the light that is captured by analyte molecules…

Q: Which of the the following is false regarding spectrophotometry?   A. The concentration of a…

A: The concentration of a sample in a solution is measured using spectrophotometer. It observes the…

Q: Question 25 Using the given data above, determine the following information: What is the…

A: As per Beer-Lambert's law:

Q: 1.2 In the food industry, food safety is a major concern. For example, the food-dye, FD&C Yellow 6,…

A: "Since there are multiple questions and it is not mentioned which one has to answer, i am solving…

Q: What is the relative concentration of the second sample?

A: A = ELC  E is absorptivity  L is length  C is concentration   Since substances and equipment are…

Q: The students conducted the assay for LDH activity using the two serum samples. Each cuvette (path…

A: The objective of the question is to calculate the enzyme activity and specific activity of two serum…

UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or  ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.