Here is the protocol for a UV-Vis spectrophotometer to detect water and chlorine-carbon. 1.Dissolve the water and chlorine-carbon compounds in a solvent, such as water. 2.Prepare a standard solution of known concentration that is similar to the sample being measured. 3.Calibrate the spectrophotometer using the standard solution. 4.Measure the absorbance of the sample using the spectrophotometer. 5.Calculate the concentration of the compounds in the sample using the calibration curve obtained from the standard solution. How is the spectrophotometer calibrated with standard solutions? When is the blank solution placed in the spectrophotmeter?
Here is the protocol for a UV-Vis spectrophotometer to detect water and chlorine-carbon. 1.Dissolve the water and chlorine-carbon compounds in a solvent, such as water. 2.Prepare a standard solution of known concentration that is similar to the sample being measured. 3.Calibrate the spectrophotometer using the standard solution. 4.Measure the absorbance of the sample using the spectrophotometer. 5.Calculate the concentration of the compounds in the sample using the calibration curve obtained from the standard solution. How is the spectrophotometer calibrated with standard solutions? When is the blank solution placed in the spectrophotmeter?
Chapter26: Molecular Absorption Spectrometry
Section: Chapter Questions
Problem 26.1QAP
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Here is the protocol for a UV-Vis spectrophotometer to detect water and chlorine-carbon.
1.Dissolve the water and chlorine-carbon compounds in a solvent, such as water.
2.Prepare a standard solution of known concentration that is similar to the sample being measured.
3.Calibrate the spectrophotometer using the standard solution.
4.Measure the absorbance of the sample using the spectrophotometer.
5.Calculate the concentration of the compounds in the sample using the calibration curve obtained from the standard solution.
How is the spectrophotometer calibrated with standard solutions? When is the blank solution placed in the spectrophotmeter?
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