Question 25 Using the given data above, determine the following information: What is the concentration of Jerry in the solution ? O 497 mM O 393 MM mM O 468 mM O 366 mM

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Question 25
Using the given data above, determine the following information:
What is the concentration of Jerry in the solution ?
497 mM
393 mM
468 mM
O 366 mM
Transcribed Image Text:Question 25 Using the given data above, determine the following information: What is the concentration of Jerry in the solution ? 497 mM 393 mM 468 mM O 366 mM
A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards
of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption
properties.
Absorbance
Wavelength Compound Ben 500 mM
Compound Jerry 500 mM
400 nm
0.137
0.136
450 nm
0.312
0.113
500 nm
0.154
0.078
550 nm
0.076
0.079
600 nm
0.227
0.148
650 nm
0.230
0.230
700 nm
0.151
0.357
750 nm
0.157
0.246
800 nm
0.154
0.154
A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced
an absorbance reading of 0.486 at the Amax of Ben, and 0.463 at the Amax of Jerry.
STD CURVE BEN
Amax Ben
Amax Jerry
STD CURVE JERRY
Aтаx Ben
Amax Jerry
CONC (mM)
CONC (mM)
ABS
ABS
ABS
ABS
100
0.074
0.025
100
0.037
0.074
200
0.148
0.057
200
0.081
0.148
400
0.284
0.103
400
0.164
0.284
800
0.607
0.218
800
0.287
0.607
1600
1.457
0.499
1600
0.678
1.457
Transcribed Image Text:A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the Amax of Ben, and 0.463 at the Amax of Jerry. STD CURVE BEN Amax Ben Amax Jerry STD CURVE JERRY Aтаx Ben Amax Jerry CONC (mM) CONC (mM) ABS ABS ABS ABS 100 0.074 0.025 100 0.037 0.074 200 0.148 0.057 200 0.081 0.148 400 0.284 0.103 400 0.164 0.284 800 0.607 0.218 800 0.287 0.607 1600 1.457 0.499 1600 0.678 1.457
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