Do your graphs show the trends a)you expected based on what you know about absorbance and transmittance using a spectrophotometer? Why or why not? b) why does absorbance data relate more conveniently to concentration that does transmission data

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter25: Instruments For Optical Spectrometry
Section: Chapter Questions
Problem 25.6QAP
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Do your graphs show the trends a)you expected based on what you know about absorbance and transmittance using a spectrophotometer? Why or why not? b) why does absorbance data relate more conveniently to concentration that does transmission data?
Experiment 1
The relationship between the concentration of flavin and its
transmission and absorbance of light.
Graph 1
100
90
80
70-
60-
transmission
50-
40-
30
20
10
0-
0.02
0.04
0.06
0.08
0.1
0.12
Flavin concentration (mM)
Graph 2
2.0-
1.8-
1.6-
1.4-
1.2-
Abso-bance
1.0-
0.8-
0.6-
0.4-
0.2-
04
0.02
0.04
0.06
0.08
0.1
0.12
Flavin concentration (mM)
13I
Transcribed Image Text:Experiment 1 The relationship between the concentration of flavin and its transmission and absorbance of light. Graph 1 100 90 80 70- 60- transmission 50- 40- 30 20 10 0- 0.02 0.04 0.06 0.08 0.1 0.12 Flavin concentration (mM) Graph 2 2.0- 1.8- 1.6- 1.4- 1.2- Abso-bance 1.0- 0.8- 0.6- 0.4- 0.2- 04 0.02 0.04 0.06 0.08 0.1 0.12 Flavin concentration (mM) 13I
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