Do your graphs show the trends a)you expected based on what you know about absorbance and transmittance using a spectrophotometer? Why or why not? b) why does absorbance data relate more conveniently to concentration that does transmission data
Do your graphs show the trends a)you expected based on what you know about absorbance and transmittance using a spectrophotometer? Why or why not? b) why does absorbance data relate more conveniently to concentration that does transmission data
Chapter25: Instruments For Optical Spectrometry
Section: Chapter Questions
Problem 25.6QAP
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Do your graphs show the trends a)you expected based on what you know about absorbance and transmittance using a spectrophotometer? Why or why not?
b) why does absorbance data relate more conveniently to concentration that does transmission data?

Transcribed Image Text:Experiment 1
The relationship between the concentration of flavin and its
transmission and absorbance of light.
Graph 1
100
90
80
70-
60-
transmission
50-
40-
30
20
10
0-
0.02
0.04
0.06
0.08
0.1
0.12
Flavin concentration (mM)
Graph 2
2.0-
1.8-
1.6-
1.4-
1.2-
Abso-bance
1.0-
0.8-
0.6-
0.4-
0.2-
04
0.02
0.04
0.06
0.08
0.1
0.12
Flavin concentration (mM)
13I
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