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1.2 In the food industry, food safety is a major concern. For example, the food-dye, FD&C Yellow 6, is approved for use in foods but the acceptable daily intake by children is 3.75 mg/kg due to concerns like hyperactivity. A UV-vis spectroscopy calibration curve for yellow dye 6 (absorbance vs. concentration in mol/L) was prepared at 485nm. The equation of the linear region between 0.2 and 1.4 absorbance is: y = 19896x Determine the molar extinction coefficient of yellow dye 6 at 485 nm. a. Assume the pathlength is 1.000 cm.

1.2 In the food industry, food safety is a major concern. For example, the food-dye, FD&C Yellow 6, is approved for use in foods but the acceptable daily intake by children is 3.75 mg/kg due to concerns like hyperactivity. A UV-vis spectroscopy calibration curve for yellow dye 6 (absorbance vs. concentration in mol/L) was prepared at 485nm. The equation of the linear region between 0.2 and 1.4 absorbance is: y = 19896x Determine the molar extinction coefficient of yellow dye 6 at 485 nm. a. Assume the pathlength is 1.000 cm.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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1.2 In the food industry, food safety is a major concern. For example, the food-dye, FD&C Yellow 6, is approved for use in foods but the acceptable daily intake by children is 3.75 mg/kg due to concerns like hyperactivity. A UV-vis spectroscopy calibration curve for yellow dye 6 (absorbance vs. concentration in mol/L) was prepared at 485nm. The equation of the linear region between 0.2 and 1.4 absorbance is: y = 19896x Determine the molar extinction coefficient of yellow dye 6 at 485 nm. a. Assume the pathlength is 1.000 cm.
Transcribed Image Text:1.2 In the food industry, food safety is a major concern. For example, the food-dye, FD&C Yellow 6, is approved for use in foods but the acceptable daily intake by children is 3.75 mg/kg due to concerns like hyperactivity. A UV-vis spectroscopy calibration curve for yellow dye 6 (absorbance vs. concentration in mol/L) was prepared at 485nm. The equation of the linear region between 0.2 and 1.4 absorbance is: y = 19896x Determine the molar extinction coefficient of yellow dye 6 at 485 nm. a. Assume the pathlength is 1.000 cm.
b. You want to use the calibration curve to estimate the concentration of yellow dye 6 in 2.00 mL samples of lemon-lime sports drink by extrapolation. You measure the following data: Sample Absorbance 1.64 1.55 A B 1.20 D 1.00 E 0.54 F 0.19 Which samples can you reliably estimate the concentration of yellow dye 6 for, if you extrapolate from the calibration curve? ) You decide to add one more pair of data points to the calibration curve using a standard stock solution of 10.00 mL yellow dye 6 that is 7.5 x 10-5 M. You remove 3.00 mL of the stock, add it to a 10.00 mL volumetric flask and make it up to the mark with de-ionized water. What is the final concentration of the solution? d. Based on your answer in c above, is the concentration low enough to extend the range of the calibration curve? Briefly explain.
Transcribed Image Text:b. You want to use the calibration curve to estimate the concentration of yellow dye 6 in 2.00 mL samples of lemon-lime sports drink by extrapolation. You measure the following data: Sample Absorbance 1.64 1.55 A B 1.20 D 1.00 E 0.54 F 0.19 Which samples can you reliably estimate the concentration of yellow dye 6 for, if you extrapolate from the calibration curve? ) You decide to add one more pair of data points to the calibration curve using a standard stock solution of 10.00 mL yellow dye 6 that is 7.5 x 10-5 M. You remove 3.00 mL of the stock, add it to a 10.00 mL volumetric flask and make it up to the mark with de-ionized water. What is the final concentration of the solution? d. Based on your answer in c above, is the concentration low enough to extend the range of the calibration curve? Briefly explain.
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