Given the information below:Amino AcidSourceBioactivitypHAspartic Acid(Asp) / DBoneEnzyme4pK1 = 2.09pK2 = 9.82рКЗ %33.86CH2Answer the following:A. Amino acids and proteins are the major buffer constituents of the blood and some bodyfluids. The amino acid is Aspartlc Acld. What will be the weak acid and conjugate base forms ofthe buffer prepared from the amino acid? (Use the pka values given).B. If you are to make 0.1 M of the buffer, what should be the molar concentration of the weakacid form and the conjugate base of the amino acid?

Amino acids are organic molecules made up of 3 functional groups such as amine, carboxyl, and…

Answered: Given the information below: Amino Acid…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Please answer the question below and show all your work. You are given a pure protein sample to characterize and provided the following information: Its molar extinction coefficient, ε280, is 0.25 liters micromole^-1 cm^-1 Using a 0.5 cm pathlength cell, you measure the absorbance at 280 nm of a 20- fold dilution of your pure protein in solution (by this, we mean that 50 ul of the protein sample was diluted to a final volume of 1 ml) and find A280 = 0.40. What is the original concentration of the protein before dilution?
Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. The total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18.4 * 10° positively charged amine groups that can adsorba negatively charged protein that passes through the column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pk, of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 5.6. 2. A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). B. After reaching 80% capacity, explain what you would do to release the protein attached to the…
For the amino acid tryptophan: Draw its complete protonic equilibria. Indicate the net charge of each form and encircle the zwitterionic form. Provide labels (A, B, C, etc.) for each form. b. Calculate the IpH of the amino acid. c. At what pH range(s) it can act as a buffer? d. What will be the predominant form(s) of the amino acid at pH (i) 1.5; (ii) 3.0; (iii) 4.5; (iv) 6.0; (v) 7.5 and (vi) 9.0?
A mixture of three proteins was subjected to ion exchange chromatography using CM-cellulsoe as the stationary phase. The elution buffer had a pH of 7.5. Proteins were eluted by increasing the ionic strength of the buffer. Predict the order of elution of the following proteins:Pepsinogen, pI = 1Cytochrome c, pI = 10.6Myoglobin, pI = 6.8
This is all part of one question. It goes together. (I wrote D and E that were cut off from the picture.) Please help me solve this since it all follows a line.D. At which pH value does the amino group of amino acid have the best buffering capacity?9.009.691.536.013.0010.5E. What is the pI (isoelectric point)?pI=
Using the equation for the best-fit straight line through your data, the average absorbance of your unknown samples U1 and U2, and any dilution factors (DON’T LEAVE OUT THE DILUTION FACTOR), calculate the concentration of protein in the original unknown protein sample. y = 1.6849x + 0.0414R² = 0.9904
In a 0.1000 M acetic acid solution at 25 degrees celsius , the acid ionizes to the extent of about 1.34 %. Since each molecule of acetic acid which ionizes produces 1 H+ ion and 1 C2H3O2- ion, the concentration in the solution are: HC2H3O2 < -----------> H+ + C2H3O2-
Diethylaminoethyl cellulose is a positively charged resin used in ion-exchange chromatography with a pKa is about 11. A negatively charged protein with an isoelectric point of 5.0 is applied to the column in a buffer at pH 7 and containing 0.1 M NaCl. Which of the following conditions is most likely to weaken the interaction between the protein and the resin? A Raising the pH to 8 and decreasing the NaCl to 0.05 M B Raising the pH to 8 and increasing the NaCl to 0.2 M C Lowering the pH to 6 and decreasing the NaCl to 0.05 M D Lowering the pH to 6 and increasing the NaCl to 0.2 M
. A protein gives, under conditions of buffer composition, pH, and tem- perature that are close to physiological conditions, a molecular weight by sedimentation equilibrium measurements of 140,000 g/mol. When the same protein is studied by SDS gel electrophoresis in the absence or presence of the reducing agent B-mercaptoethanol (BME), the pat- terns seen, respectively, in lanes A and B are observed. Lane C contains standards of molecular weight indicated. From these data, describe the native protein, in terms of the kinds of subunits present, the stoi- chiometry of subunits, and the kinds of bonding (covalent, noncova- lent) existing between subunits. / A B - BME + BME STD Serum albumin, 67,000 Ovalbumin, 43,000 Carbonic anhydrase, 30,000 Trypsin inhibitor, 20,000 10 2. 4. Co cm migrated
A researcher prepares 100 mL of aqueous solution containing 0.1 g of a protein. The researcher then uses an experimental apparatus ,where he notes that, at room temperature (300K) and atmospheric pressure (101.325 kPa), distilled water flows naturally into the solution compartment. However, when he applies an extra pressure of 1000 Pa he observes that the flow of distilled water ceases and the system is in balance. Calculate the molar mass of this protein, in kDa (1 Da = 1 g/ mol); Why does the flow of distilled water cease with the application of the pressure of 1000 Pa? Explain in terms of potential chemicals. %3D
Please draw titration curves for peptide HFDTYA and HEKDGQ, and calculate the pl for each. Use this information to explain why the peptide HFDTYA has low solubility and HEKDGQ has a higher solubility in bulk water at pH = 7.
Given a stock protein solution with a concentration of 6 mg/ml, determine the protein concentration of a solution made by mixing 5 μl of the stock with 5 μl of a buffer.

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