Mixtures of amino acids can be analyzed by first separating the mixture into its components through ion‑exchange chromatography. Amino acids placed on a cation‑exchange resin containing sulfonate (−SO−3)(−SO3−) groups flow down the column at different rates because of two factors that influence their movement: (1) ionic attraction between the sulfonate residues on the column and positively charged functional groups on the amino acids, and (2) aggregation of nonpolar amino acid side chains with the hydrophobic backbone of the polystyrene resin. Note that the ionic attraction is more important than hydrophobicity for this column media. For each pair of amino acids, identify which will be eluted first from a cation‑exchange column using a pH 7.0pH 7.0 buffer.
Mixtures of amino acids can be analyzed by first separating the mixture into its components through ion‑exchange chromatography. Amino acids placed on a cation‑exchange resin containing sulfonate (−SO−3)(−SO3−) groups flow down the column at different rates because of two factors that influence their movement: (1) ionic attraction between the sulfonate residues on the column and positively charged functional groups on the amino acids, and (2) aggregation of nonpolar amino acid side chains with the hydrophobic backbone of the polystyrene resin. Note that the ionic attraction is more important than hydrophobicity for this column media. For each pair of amino acids, identify which will be eluted first from a cation‑exchange column using a pH 7.0pH 7.0 buffer.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Mixtures of amino acids can be analyzed by first separating the mixture into its components through ion‑exchange chromatography.
Amino acids placed on a cation‑exchange resin containing sulfonate (−SO−3)(−SO3−) groups flow down the column at different rates because of two factors that influence their movement: (1) ionic attraction between the sulfonate residues on the column and positively charged
Note that the ionic attraction is more important than hydrophobicity for this column media.
For each pair of amino acids, identify which will be eluted first from a cation‑exchange column using a pH 7.0pH 7.0 buffer.
Transcribed Image Text:glutamate and lysine
arginine and methionine
aspartate and valine
glycine and leucine
serine and alanine
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