QUESTION 2You obtained the following raw data when setting up aBiuret standard curve:AbsorbancyBSA (mg/ml)540nm0.15810.21020.2600.30540.3600.4100.45570.51080.5309.0.550100.554After blanking against a biuret-dH20 sample, theprotein concentration of an unknown sample wasdetermined using thesamemethod andanabsorbancy of 0.324 was obtained.curve, excluding outliers(experimental and statistical) and determine theprotein concentration in the unknown sample in mg /Set upastandardml (up to 3 significant figures).5

The determination of protein concentration is a fundamental technique in biochemical analysis.

Answered: BSA (mg/ml) Absorbancy 540nm 0.158 1…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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