Full question provided for context, please only answer part 2 Question 3 Every year, millions of children go blind because of vitamin A deficiency. Humans cannot produce vitamin A, thus they must consume vitamin A or a vitamin A precursor such as betacarotene. You want to create a yeast strain that contains the entire beta-carotene pathway so that you can study the biochemistry of beta-carotene and vitamin A synthesis. You
Full question provided for context, please only answer part 2
Question 3
Every year, millions of children go blind because of vitamin A deficiency. Humans cannot
produce vitamin A, thus they must consume vitamin A or a vitamin A precursor such as betacarotene.
You want to create a yeast strain that contains the entire beta-carotene pathway
so that you can study the biochemistry of beta-carotene and vitamin A synthesis. You
discovered a yeast strain that contains the majority of the enzymes required for beta-carotene
production.
1. You are tasked with supplying the missing enzyme, β-carotene 15,15′-
dioxygenase (BCMO) to these yeast cells. Design a DNA cloning workflow for
the expression of the BCMO gene in these yeast cells. In your response, you
should also explain and provide the overall principles of these techniques.
2. Suggest a technique you could use to confirm that you have the correct BCMO
protein in these yeast cells. Provide reasons for your choice and describe its
principle in detail.
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