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4. RNAse A cleaves the phosphodiester backbone of RNA. Draw its mechanism.

4. RNAse A cleaves the phosphodiester backbone of RNA. Draw its mechanism.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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please answer question #4 with a handwritten mechanism
### Transcription for Educational Purposes 3. a) Sketch a Lineweaver-Burk (LB) plot with clearly labeled axes and axis units. b) Find the Vmax and Km values for this reaction. c) Show a line on this plot for MC50, which is a competitive inhibitor. 4. RNAse A cleaves the phosphodiester backbone of RNA. Draw its mechanism. ### Explanation - **Lineweaver-Burk Plot (LB Plot):** A graphical representation used to illustrate the kinetics of enzyme-catalyzed reactions. This plot is a double reciprocal graph based on the Michaelis-Menten equation, often used to determine important parameters like Vmax (maximum reaction velocity) and Km (Michaelis constant). - **Competitive Inhibitor:** A molecule that competes with the substrate for binding to the enzyme active site. In a Lineweaver-Burk plot, the presence of a competitive inhibitor would be shown as a change in the slope of the line, indicating an increase in Km while Vmax remains unchanged. - **RNAse A Mechanism:** This is an enzymatic process by which RNAse A cleaves RNA molecules. The enzyme attacks the phosphodiester bonds within RNA, resulting in the breakdown of the RNA strand. Students are asked to draw the detailed mechanism, which typically involves the formation of an enzyme-substrate complex and the catalytic process leading to cleavage. This transcription is suitable for educational contexts where students are learning about enzyme kinetics and RNA enzymology.
Transcribed Image Text:### Transcription for Educational Purposes 3. a) Sketch a Lineweaver-Burk (LB) plot with clearly labeled axes and axis units. b) Find the Vmax and Km values for this reaction. c) Show a line on this plot for MC50, which is a competitive inhibitor. 4. RNAse A cleaves the phosphodiester backbone of RNA. Draw its mechanism. ### Explanation - **Lineweaver-Burk Plot (LB Plot):** A graphical representation used to illustrate the kinetics of enzyme-catalyzed reactions. This plot is a double reciprocal graph based on the Michaelis-Menten equation, often used to determine important parameters like Vmax (maximum reaction velocity) and Km (Michaelis constant). - **Competitive Inhibitor:** A molecule that competes with the substrate for binding to the enzyme active site. In a Lineweaver-Burk plot, the presence of a competitive inhibitor would be shown as a change in the slope of the line, indicating an increase in Km while Vmax remains unchanged. - **RNAse A Mechanism:** This is an enzymatic process by which RNAse A cleaves RNA molecules. The enzyme attacks the phosphodiester bonds within RNA, resulting in the breakdown of the RNA strand. Students are asked to draw the detailed mechanism, which typically involves the formation of an enzyme-substrate complex and the catalytic process leading to cleavage. This transcription is suitable for educational contexts where students are learning about enzyme kinetics and RNA enzymology.
Expert Solution
Step 1

INTRODUCTION :

RNA - It is called Ribonucleic acid, It is a nucleic acid which is present in all living cells which has structural similarities to DNA(deoxyribo nucleic acid),but unlike DNA,RNA is mostly a single stranded structure.

There are mainly 3 types of RNA - 1) mRNA(messenger RNA)

                                                        2) rRNA (ribosomal RNA)

                                                         3) tRNA( transfer RNA)

 

RNase A : It is a pancreatic ribonuclease enzyme which cleaves single stranded RNA. RNase A is a relatively small protein,having around 124 residues.

 

 

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