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RNase Q is an enzyme that has identical activity to RNase A, but is found only in a group of humans who are unusually susceptible to highly implausible conspiracy theories. The sequence of RNase Q differs from RNase A in that all 8 Cys residues are substituted with Ala. However, the three-dimensional structure of RNase Q is the same as that of RNase A, and the enzymatic activity is the same. If RNase Q is unfolded in the presence of a high concentration of urea, all of the secondary and tertiary structure is disrupted, and it can no longer catalyze the destruction of RNA. If you then slowly remove the urea, RNase Q can refold, and 100% of its activity returns. (a) Based on these experimental observations, can you conclude that the amino acid sequence of RNase Q dictates one unique three-dimensional fold of the protein? Why or why not?

RNase Q is an enzyme that has identical activity to RNase A, but is found only in a group of humans who are unusually susceptible to highly implausible conspiracy theories. The sequence of RNase Q differs from RNase A in that all 8 Cys residues are substituted with Ala. However, the three-dimensional structure of RNase Q is the same as that of RNase A, and the enzymatic activity is the same. If RNase Q is unfolded in the presence of a high concentration of urea, all of the secondary and tertiary structure is disrupted, and it can no longer catalyze the destruction of RNA. If you then slowly remove the urea, RNase Q can refold, and 100% of its activity returns. (a) Based on these experimental observations, can you conclude that the amino acid sequence of RNase Q dictates one unique three-dimensional fold of the protein? Why or why not?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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RNase Q is an enzyme that has identical activity to RNase A, but is found only in a group of humans who are unusually susceptible to highly implausible conspiracy theories. The sequence of RNase Q differs from RNase A in that all 8 Cys residues are substituted with Ala. However, the three-dimensional structure of RNase Q is the same as that of RNase A, and the enzymatic activity is the same. If RNase Q is unfolded in the presence of a high concentration of urea, all of the secondary and tertiary structure is disrupted, and it can no longer catalyze the destruction of RNA. If you then slowly remove the urea, RNase Q can refold, and 100% of its activity returns. (a) Based on these experimental observations, can you conclude that the amino acid sequence of RNase Q dictates one unique three-dimensional fold of the protein? Why or why not? (b) Can you also conclude that this one unique three-dimensional fold of the protein is required for catalytic activity? Why or why not?
Transcribed Image Text:RNase Q is an enzyme that has identical activity to RNase A, but is found only in a group of humans who are unusually susceptible to highly implausible conspiracy theories. The sequence of RNase Q differs from RNase A in that all 8 Cys residues are substituted with Ala. However, the three-dimensional structure of RNase Q is the same as that of RNase A, and the enzymatic activity is the same. If RNase Q is unfolded in the presence of a high concentration of urea, all of the secondary and tertiary structure is disrupted, and it can no longer catalyze the destruction of RNA. If you then slowly remove the urea, RNase Q can refold, and 100% of its activity returns. (a) Based on these experimental observations, can you conclude that the amino acid sequence of RNase Q dictates one unique three-dimensional fold of the protein? Why or why not? (b) Can you also conclude that this one unique three-dimensional fold of the protein is required for catalytic activity? Why or why not?
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