E23. In the Western blot shown here, proteins were isolated from red blood cells and muscle cells from two different individuals. One individual was unaffected, and the other suffered from a disease known as thalassemia, which involves a defect in hemoglobin. The blot was exposed to an antibody that recognizes B globin, which is one of the polypeptides that constitute hemoglobin. Equal amounts of total cellular proteins were added to each lane. Lane 1: Proteins isolated from normal 2 3 4 red blood cells Lane 2: Proteins isolated from the red blood cells of a thalassemia patient Lane 3: Proteins isolated from normal muscle cells Lane 4: Proteins isolated from the muscle cells of a thalassemia patient Explain these results.
E23. In the Western blot shown here, proteins were isolated from red blood cells and muscle cells from two different individuals. One individual was unaffected, and the other suffered from a disease known as thalassemia, which involves a defect in hemoglobin. The blot was exposed to an antibody that recognizes B globin, which is one of the polypeptides that constitute hemoglobin. Equal amounts of total cellular proteins were added to each lane. Lane 1: Proteins isolated from normal 2 3 4 red blood cells Lane 2: Proteins isolated from the red blood cells of a thalassemia patient Lane 3: Proteins isolated from normal muscle cells Lane 4: Proteins isolated from the muscle cells of a thalassemia patient Explain these results.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:E23. In the Western blot shown here, proteins were isolated from red
blood cells and muscle cells from two different individuals. One
individual was unaffected, and the other suffered from a disease
known as thalassemia, which involves a defect in hemoglobin. The
blot was exposed to an antibody that recognizes B globin, which is
one of the polypeptides that constitute hemoglobin. Equal amounts
of total cellular proteins were added to each lane.
Lane 1: Proteins isolated from normal
2
3
4
red blood cells
Lane 2: Proteins isolated from the red
blood cells of a thalassemia
patient
Lane 3: Proteins isolated from normal
muscle cells
Lane 4: Proteins isolated from the
muscle cells of a thalassemia
patient
Explain these results.
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