Cell cultures can be synchronized, or brought into the same phase of the cellcycle, by various means. For example, adding thymidine to a cell culturecauses all cells to become arrested early in S phase. What is the mechanismby which thymidine treatment blocks DNA replication?

Cell cycle is an accurate process in which cell grows in the G1 phase, duplicates its DNA in S…

Answered: Cell cultures can be synchronized, or…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The experiment below is from a seminal set of experiments in the 1960s that illustrated the role of various repair pathways for DNA damage caused by UV radiation. In this experiment, the scientists isolated E coli strains that are mutant in the Rec A gene, the UvrA gene or both. They then irradiated cultures of each strain with increasing doses of UV light and measured the effect on cell viability. Answer the following questions about this data. A. Which DNA repair pathway and repair activity is inhibited by the Rec A mutant? B. Which DNA repair pathway and repair function is inhibited by UvrA mutant? C. Why is the UvrA/RecA double mutant so much more senitive to UV light than either mutant alone?
Deamination of cytosine to form uracil can happen spontaneously during replication. It can be repaired relatively easily by photoreactivation repair using DNA polymerase I b) base excision repair using DNA glycosylase creating an AP site c) base excision repair using topoisomerase creating an AP site d) none of these
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2b) Suppose you generated a mutant E. coli strain in which it was DNA Polymerase I that was inactivated - assuming that all the other enzymes involved in replication remained fully functional, how would DNA replication in these mutant cells lacking DNA Pol I differ from DNA replication in normal E. coli? Briefly explain why you would expect to see that change/those changes in DNA replication in the mutant cells.
Describe a typical program used for DNA amplification with the thermal cycler and the purpose of each steps.
In the Hershey Chase experiment, why was the localization of radio-labeled phage of macromolecules in the cytoplasm the reason that the genetic material identity was proved.
Which of the following statements is/are TRUE about DNA repair? Question 24 options: Ensure no mutations to ever occur in a cell. May occur in dividing and non-dividing cells. May involve in the synthesis of new DNA. May be error prone.
Yeast cells are eukaryotes, and they require a functional secretory pathway to grow and to maintain cellular organization. But surprisingly, when SRP is removed from yeast cells by deleting the relevant genes, the cells are still alive (although they grow slowly). a) How can yeast survive without SRP? Propose two alternative hypotheses. b) How might electron microscopy of normal and SRP-deficient yeast cells help you to distinguish between these two hypotheses?
why is it more advantageous for the cell to have its DNA fused to the cell wall as opposed to dispersed freely in the cell?
Consider a hypothetical phage whose DNA replicates exclusively by rolling circle replication. A phage with radioactive DNA in both strands infects a bacterium and is allowed to replicate in a nonradioactive medium. Assume that only daughter DNA from the elongated branch ever gets packaged into progeny particles. What fraction of the parental radioactivity will appear in the progeny phage? How many progeny phage will contain radioactive DNA? What is the fundamental difference between the initiation of θ replication and that of the rolling circle?
As shown , several medical agents are now commercially produced by genetically engineered microorganisms. Discuss the advantages and disadvantages of making these agents this way.

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Cell cultures can be synchronized, or brought into the same phase of the cell cycle, by various means. For example, adding thymidine to a cell culture causes all cells to become arrested early in S phase. What is the mechanism by which thymidine treatment blocks DNA replication?