Calculate a) the number of theoretical plates and b) the plate height for a peak with a retention time of 1295 seconds, a width at the baseline of 27 seconds and elutes from a 15 m column, the solvent peak is at 60 seconds. If the second peak is at 1540 seconds with a width of 28 seconds. Calculate c) the selectivity and d) resolution.

Calculate a) the number of theoretical plates and b) the plate height for a peak with a retention time of 1295 seconds, a width at the baseline of 27 seconds and elutes from a 15 m column, the solvent peak is at 60 seconds. If the second peak is at 1540 seconds with a width of 28 seconds. Calculate c) the selectivity and d) resolution.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.17QAP: From the data in Problem 26-14, calculate for species C and D) (a) the resolution. (b) the length of...

See similar textbooks

Similar questions

From the data in Problem 26-14, calculate for species C and D) (a) the resolution. (b) the length of column necessary to separate the two species with a resolution of 2.5.
Retention time is the time taken for the mobile phase to reach the detector. True False
The chromatogram in the figure is of a mixture of 2 compounds, carried out in a packed liquid chromatographic column of 25 cm length. The flow rate was 0.40 mL min ¹. A species that is not retained passes through the column in 5 minutes. a) Determine the retention factors for the two components. b) Calculate the resolution of the two peaks. c) Find the average height of the plate. d) What column length would be needed to achieve a resolution of 1.75? Detector response 0 5.0 10 22.0 20 30.0 30 Time, min 38.0 40 39.0 50 50.0 60 62.0 70
Using smaller particle size for stationary phase in HPLC columns results in a(n) a) decrease in the retention times, t, b) Increase in the resolution, R c) Increase in the plate height, H d) Decrease the required pressure P
Substances A and B have retention times of 16.40 and 17.63 minutes, in a 30.0 cm column. A species that is not retained passes through the column in 1.30 minutes. The peak widths (at the base) for A and B are 1.11 and 1.21 minutes.Calculate:a) Resolution of the columnb) Average number of plates in the column.c) Height of the plate.d) Time required to elute substance B in a column that gives a resolution value, Rs = of 1.5.
Please solve athe question bellow
What is the role of specific visualizing agents in a Thin layer Chromatography? Please shortly answer at your own words. Answer should be to the point (4-5 lines).
1,9
3 questions...
In molecular exclusion (size exclusion) chromatography, the molecules have the longest retention times. a) the larger molecules b) the smaller molecules
A sample that contained two components was separated on a column. The column had a length of 15.0 cm. Unfortunately, these two components were not well resolved, with a resolution of 1.2. For getting a resolution of 2.0, what column length should be used? Please notes: Assume that the separation factor and retention factors do not change greatly.
The retention times of two compounds A and B are 16.40 and 17.63 minutes. A species that is not retained passes through the column in 1.30 minutes. The peak widths (at the base) for A and B are 1.11 and 1.21 minutes, respectively. Calculate:a) The resolution of the columnb) The resolution between compounds A and B