Two substances A and B have retention times of 16.40 and 17.63 min respectively on 30.0 cm column. An unsuccessful species passed over the column in 1.30 min. The widths (at the base) of the peaks ofA and B are respectively 1.11 min and 1.21 min: Calculate: a) The Resolution Rs of the column. b) The average number (Nav of theoretical plates in the column c) The equivalent height (H) d) length of column required to achieve a resolution of 1.5, e) time required to elute substance B on the longer column.

Two substances A and B have retention times of 16.40 and 17.63 min respectively on 30.0 cm column. An unsuccessful species passed over the column in 1.30 min. The widths (at the base) of the peaks ofA and B are respectively 1.11 min and 1.21 min: Calculate: a) The Resolution Rs of the column. b) The average number (Nav of theoretical plates in the column c) The equivalent height (H) d) length of column required to achieve a resolution of 1.5, e) time required to elute substance B on the longer column.

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter31: Introduction To Analytical Separations

Section: Chapter Questions

Problem 31.32QAP

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Question

Two substances A and B have retention times of

16.40 and 17.63 min respectively on 30.0 cm column. An

unsuccessful species passed over the column in 1.30 min.

The widths (at the base) of the peaks ofA and B are

respectively 1.11 min and 1.21 min: Calculate:

a) The Resolution Rs of the column.

b) The average number (Nav of theoretical plates in the

column

c) The equivalent height (H)

d) length of column required to achieve a resolution of 1.5,

e) time required to elute substance B on the longer column.

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