The following data are for a liquid chromatographic column: column length 24.7 cm volume of the mobile phase, Vm 1.37 mL volume of the stationery phase, Vs 0.164 mL A chromatogram of a mixture of species A, B, C and D provided the following data: Calculate the plate height in milimeters (mm) for species A. Retention Widht of Peak Time, min Base (W), min Nonretained 3.1 5.4 0.41 B 13.3 1.07 14.1 1.16 D 21.6 1.72

The following data are for a liquid chromatographic column: column length 24.7 cm volume of the mobile phase, Vm 1.37 mL volume of the stationery phase, Vs 0.164 mL A chromatogram of a mixture of species A, B, C and D provided the following data: Calculate the plate height in milimeters (mm) for species A. Retention Widht of Peak Time, min Base (W), min Nonretained 3.1 5.4 0.41 B 13.3 1.07 14.1 1.16 D 21.6 1.72

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Two solutes have an unadjusted retention of γ = 1.06. How many plates are required to give a resolution of 1.5? Of 3.0? While we did not discuss this in class, the number of plates can also be expressed as N = L/H where H is plate height and L is the length of the entire column. If the plate height is 0.5 mm in gas chromatography, how long must the column be for a resolution of 1.5? If γ = 1.06 and H = 3 microns in liquid chromatography, what length of column in cm gives a resolution of 1.5?
From the figure, which represents the chromatographic peak of a compound A, calculate the chromatographic parameters: a) Number of theoretical plates for compound A. b) The theoretical plate height, taking into account that it is a fused silica column of 0.25 mm x 30 m in diameter and column length. c) The resolution of this compound with respect to a compound B, which appears at a retention time of 18.1 min, which begins to elute at minute 18.02 and which ends its elution at minute 18.2. total ionic current (%) 18 18.2 18.4 18.75 19.0 19.25 18.6 18.8 19 19.2 19.4 19.6 19.8 20 time (min)
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1. A two-component mixture is analyzed by paper TLC. Component A is more soluble in the mobile phase than component B. The following result is obtained. Calculate the Rifor each component and label the identity of each Frond (3-0,4)CM3D2.6cmr A (2-6-04 m = 2.2cm B C67-0.ycm=0.3cm RF (A= 2.2cm = 0.85 2. Lem spot. 2 39で Solvent Front A0-2.6m %3D 3cm Spoting Line Rf (8- 0.3cm 2. Scm = 0.12 %3D 2. Which one is the more polar component? Explain IS morC polo Because its Rf Is low 3. If a more polar solvent (mgbil phase) is used for the above experiment, the result will be: O Both component A and Component B will travel further up the plate-Distance may verur b) Only Component A will travel further up the plat c) Only Component B will travel further up the plat d) They will travel on the plate the same distance as when a less polar Choose one of the answers and explain 4. Paper chromatography works as the same principle as TLC however, cellulose paper (the same as filter paper) is used for…
Consider the following method for the analysis of cocaine (MW 303.35) powder. "Dissolve about 600 mg of cocaine, preciously dried and accurately weighed, in 50 mL of glacial acetic acid, add I drop of crystal violet TS, and titrate with 0.1 M perchloric acid VS to a green endpoint. Perform a blank determination, and make the necessary correction" Calculate the percentage purity of the cocaine sample from the data below. Show your reasoning clearly and record your final answer to four significant figures. CH₂ ● Cocaine powder weighed = 606.3 mg Concentration of HCIO3=0.09978 M H₂C-N- Volume HCIO3 required for the assay mixture = 19.97 mL ● Volume HCIO; required for blank determination = 0.059'mL
You perform a concentration of a single component using displacement chromatography, and the following data applies: Concentration of binding sites: 4*10^(-4) M Kd for displacer: 2.0*10^(-5) M Kd for component A: 4*10^(-5) M Kd for component B: 1.5*10^(-4) M The concentration of the injected displacer is 2.0*10^(-4) M Determine and justify whether the components are part of a displacement system, and if so, provide their free and bound concentrations. Assume that the number of binding sites is the same for all components.
Protein ABC is a heme-containing protein responsible in electron transfer in microalgae. The target protein was extracted using ammonium sulfate fractional precipitation and quantified using Bradford assay. A set of solutions with increasing concentration was prepared using 15.00 mg/mL analogous protein standard [refer to succeeding table]. Each solution was treated with 80 μL of Bradford reagent and diluted to 1.500mL. The absorbance at 595 nm of each solution was obtained and presented below. Volume of protein std, μL A595 0 0.0002 10 0.0007 25 0.0075 50 0.0165 100 0.0356 250 0.0930 500 0.1950 750 0.3010 1000 0.4175 Report the equation of the calibration curve and proof of linearity (R2 value).
In order to improve the peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following:“2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm3 in a graduated flask. To this solution, 50 cm3 of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm3 min-1.”Based on the given preparation procedure, identify THREE mistakes that were made.
4. The total retention time of dodecane on a 15 m x 0.25 mm i.d. DB - 5 GC column was 8.0 min . The column efficiency according to this peak as 25,600 total theoretical plates. The peak had this width at the base :
In a 1.10 m gas-liquid chromatographic column the following results were obtained: air, 18.0 s; methyl acetate, 1.98 min.; methyl propionate, 2.24 min; methyl n-butyrate, 7.93 min. The base widths of the last three peaks were: 0.19; 0.23 and 0.79 min, respectively. Calculate the theoretical number of plates and the plate height of each column.
The following statements are true for any chromatographic analysis except: The analyte component interacting most strongly with the stationary phase elute faster than those with weaker interactions. On the other hand, those with least interaction with the mobile phase elute the slowest. Interactions between mobile phase-analyte - stationary phase can be both chemical and physical in nature. Chromatography is a separation technique. XPS can be used for the following analyses except-* Contamination in the surface or the bulk of the sample Functional group of the sample Binding energy of electronic states Thickness of materials Density of electronic states A technique used in observing trace elements, isotopes and atomic monolayers based on the secondary ions that are ejected from the sample. XRD AFM SEM SIMS ELLIPSOMETRY XPS NLO
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