The following data are for a liquid chromatographic column: column length 24.7 cm volume of the mobile phase, Vm 1.37 mL volume of the stationery phase, Vs 0.164 mL A chromatogram of a mixture of species A, B, C and D provided the following data: Calculate the plate height in milimeters (mm) for species A. Retention Widht of Peak Time, min Base (W), min Nonretained 3.1 5.4 0.41 B 13.3 1.07 14.1 1.16 D 21.6 1.72

The following data are for a liquid chromatographic column: column length 24.7 cm volume of the mobile phase, Vm 1.37 mL volume of the stationery phase, Vs 0.164 mL A chromatogram of a mixture of species A, B, C and D provided the following data: Calculate the plate height in milimeters (mm) for species A. Retention Widht of Peak Time, min Base (W), min Nonretained 3.1 5.4 0.41 B 13.3 1.07 14.1 1.16 D 21.6 1.72

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Substances A, B, C and D were found to have retention times of 8.3 min (A), 10.6 min (B), 12.4 min (C) and 13.5 min (D), respectively on an 15.0 cm long column. There was also an unretained species which appeared at 1.5 min. The widths of the peak bases were 0.90 min (A), 1.1 min (B), 1.3 min (C) and 0.80 min (D). Given this information listed above, please choose the most appropriate answer for the six questions listed below for the list of possible answers. Part A Calculate the capacity factor (k') for peak B and peak D. Part B. Calculate the resolution (R) between peaks B and D. Part C. What do the capacity factor and resolution tell you about the separation of peaks B and D on this column? Part D What are the theoretical plate number (N) for peaks Band D? Part E. What is the average plate height (H), based on the information in Part D and the column length listed above? Part F What would be the required length of a column to achieve a Rs of 2.07
You perform a concentration of a single component using displacement chromatography, and the following data applies: Concentration of binding sites: 4*10^(-4) M Kd for displacer: 2.0*10^(-5) M Kd for component A: 4*10^(-5) M Kd for component B: 1.5*10^(-4) M The concentration of the injected displacer is 2.0*10^(-4) M Determine and justify whether the components are part of a displacement system, and if so, provide their free and bound concentrations. Assume that the number of binding sites is the same for all components.
Protein ABC is a heme-containing protein responsible in electron transfer in microalgae. The target protein was extracted using ammonium sulfate fractional precipitation and quantified using Bradford assay. A set of solutions with increasing concentration was prepared using 15.00 mg/mL analogous protein standard [refer to succeeding table]. Each solution was treated with 80 μL of Bradford reagent and diluted to 1.500mL. The absorbance at 595 nm of each solution was obtained and presented below. Volume of protein std, μL A595 0 0.0002 10 0.0007 25 0.0075 50 0.0165 100 0.0356 250 0.0930 500 0.1950 750 0.3010 1000 0.4175 Report the equation of the calibration curve and proof of linearity (R2 value).
In order to improve the peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following:“2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm3 in a graduated flask. To this solution, 50 cm3 of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm3 min-1.”Based on the given preparation procedure, identify THREE mistakes that were made.
4. The total retention time of dodecane on a 15 m x 0.25 mm i.d. DB - 5 GC column was 8.0 min . The column efficiency according to this peak as 25,600 total theoretical plates. The peak had this width at the base :
In a 1.10 m gas-liquid chromatographic column the following results were obtained: air, 18.0 s; methyl acetate, 1.98 min.; methyl propionate, 2.24 min; methyl n-butyrate, 7.93 min. The base widths of the last three peaks were: 0.19; 0.23 and 0.79 min, respectively. Calculate the theoretical number of plates and the plate height of each column.
The following statements are true for any chromatographic analysis except: The analyte component interacting most strongly with the stationary phase elute faster than those with weaker interactions. On the other hand, those with least interaction with the mobile phase elute the slowest. Interactions between mobile phase-analyte - stationary phase can be both chemical and physical in nature. Chromatography is a separation technique. XPS can be used for the following analyses except-* Contamination in the surface or the bulk of the sample Functional group of the sample Binding energy of electronic states Thickness of materials Density of electronic states A technique used in observing trace elements, isotopes and atomic monolayers based on the secondary ions that are ejected from the sample. XRD AFM SEM SIMS ELLIPSOMETRY XPS NLO
hree components in a mixture have varying distribution constants between the mobile phase and the stationary phass One of the components has a very low distribution constant, Would this component likely elute first or last and how would you design an experiment or modify the components of the separation to make it perform in the opposite manner?
The following data are for a GLC column: L= 35.5 cm, flow rate = 0.303 mL/min A chromatogram of a mixture of the following compounds provide the following data: (min) (min) Air 0.9 | Benzene 5.0 0.71 Toluene 7.9 0.82 Xylene 8.4 1.16 Calculate: a. The number of plates from each peak. b. The mean for N. C. The plate height H. From the data above, calculate the resolution for d. Benzene and toluene. e. Toluene and xylene. F. Benzene and xylene. g. The length of column required to achieve an average resolution of 4.50.
Consider the following chromatographic data for the separation of methylparaben from ethylparaben in a sunscreen sample. Compound Methylparaben Retention time, tr (min). Width at base of peak, Wb (min) 3.2 4.2 0.55 0.60 Ethylparaben A compound not retained by the column in this analysis had a tm of 0.75 minutes. The column used was a C18 column with a mobile phase mixture of 75% methanol and 25% water. Calculate the number of theoretical plates for ethylparaben.
In a HPLC separation of two compounds, A and B, the retention times observed are 3.5 min. for A and 4.5 min. for B on a 15 cm column. The retention time for the mobile phase is 1.0 min. The peak widths (at the base) for A and B are 0.5 and 0.7 min respectively. Calculate the following: (i) The capacity factor of A and B. (ii) The peak resolution. (iii) The average number of theoretical plates in the column