In a High Performance Liquid Chromatography (HPLC) analysis, toluene exhibits a capacity factor of 3.5 on a 15.0 cm column. The unretained solute is eluted at 1.33 min (tm). a) Determine the retention time tR of toluene. b) Calculate the number of theoretical plates and the plate height that would produce atoluene peak eluting at 12.83 min with a width at the base of 18.4 s

In a High Performance Liquid Chromatography (HPLC) analysis, toluene exhibits a capacity factor of 3.5 on a 15.0 cm column. The unretained solute is eluted at 1.33 min (tm). a) Determine the retention time tR of toluene. b) Calculate the number of theoretical plates and the plate height that would produce atoluene peak eluting at 12.83 min with a width at the base of 18.4 s

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.14QAP

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Question

In a High Performance Liquid Chromatography (HPLC) analysis, toluene exhibits a capacity
factor of 3.5 on a 15.0 cm column. The unretained solute is eluted at 1.33 min (tm).

a) Determine the retention time tR of toluene.

b) Calculate the number of theoretical plates and the plate height that would produce
atoluene peak eluting at 12.83 min with a width at the base of 18.4 s

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