Consider the peaks for pentafluorobenzene and benzene in the chromatogram shown below. The elution time of the unretained solute is 1.06 min. The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a stationary phase 3.0 μm thick on the inner wall. (a) Find the adjusted retention times and capacity factors for both compounds; (b) Find the relative retention (also known as selectivity factor) between the two compounds; (c) Measuring W₁/2 on the chromatogram, find the number of plates (N₁ and N₂) and the plate height for these two compounds. (d) Measuring the width (w) at the baseline on the chromatogram, find the number of plates for these two compounds and compare to your answer for (c); (e) Using the width measurements from (c) to derive the baseline widths for the two compounds, find the resolution between the two peaks;

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Answer the questions by using the given graph below

Detector response
H
LL
F
F
F
F
12.98 min
13.00
13.20 min
t
13.81 min
13.50
Time (min)
F
14.77 min
n-C₂H16
14.00
14.56 min
14.50
JL
Transcribed Image Text:Detector response H LL F F F F 12.98 min 13.00 13.20 min t 13.81 min 13.50 Time (min) F 14.77 min n-C₂H16 14.00 14.56 min 14.50 JL
Consider the peaks for pentafluorobenzene and benzene in the chromatogram shown below. The
elution time of the unretained solute is 1.06 min. The open tubular column is 30.0 m in length and
0.530 mm in diameter, with a stationary phase 3.0 μm thick on the inner wall.
(a) Find the adjusted retention times and capacity factors for both compounds;
(b) Find the relative retention (also known as selectivity factor) between the two compounds;
(c) Measuring W₁/2 on the chromatogram, find the number of plates (N₁ and N₂) and the plate height
for these two compounds.
(d) Measuring the width (w) at the baseline on the chromatogram, find the number of plates for
these two compounds and compare to your answer for (c);
(e) Using the width measurements from (c) to derive the baseline widths for the two compounds,
find the resolution between the two peaks;
Transcribed Image Text:Consider the peaks for pentafluorobenzene and benzene in the chromatogram shown below. The elution time of the unretained solute is 1.06 min. The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a stationary phase 3.0 μm thick on the inner wall. (a) Find the adjusted retention times and capacity factors for both compounds; (b) Find the relative retention (also known as selectivity factor) between the two compounds; (c) Measuring W₁/2 on the chromatogram, find the number of plates (N₁ and N₂) and the plate height for these two compounds. (d) Measuring the width (w) at the baseline on the chromatogram, find the number of plates for these two compounds and compare to your answer for (c); (e) Using the width measurements from (c) to derive the baseline widths for the two compounds, find the resolution between the two peaks;
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