1) Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution time for unretained solute is 1.06 min. The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a layer of stationary phase 3.0 μm thick on the inner wall. a) Find the adjusted rentention times and rentention factors for both compounds. b) Find the relative rentention c) Measuring the w1/2 on the chromatrogram , find the number of plates, N1 and N2, and the plate height for these two compounds. 14.77 minn-C,Hie14.56 min12.98 min13.20 min13.81 min13.0013.50Time (min)14.0014.50 TABLE 23-2 Summary of chromatography equations| QuantityPartition coefficientEquationParametersK= cJc.c, = concentration of solute in stationary phaseCm = concentration of solute in mobile phase1, = retention time of solute of interest= retention time of unretained solute4, = volume flow rate = volume per unit timeV, = volume of stationary phaseV = volume of mobile phase1, = time solute spends in stationary phase = 1,- 1., = time solute spends in mobile phaseAdjusted retention timeRetention volumeRetention factorV, =1, 4,k = r.- KV/VmRelative retention (alsoa =Subscripts I and 2 refer to two solutescalled separation factor)w = width at basewin = width at half-height165.55Number of platesN=winPlate heightG = standard deviation of bandH =x= distance traveled by center of bandL = length of columnN= number of plates on columnNAr,AV.Ar, = difference in retention timesAV, = difference in retention volumesW = average width measured at baseline in same unitsas numerator (time or volume)ResolutionResolutionVN (a - 1)N= number of platesa = relative retentionk = retention factor for second peakResolution =(1 + k2

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Answered: 1) Consider the peaks for…

Chemistry

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ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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1) Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution time for unretained solute is 1.06 min. The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a layer of stationary phase 3.0 μm thick on the inner wall.

a) Find the adjusted rentention times and rentention factors for both compounds.

b) Find the relative rentention

c) Measuring the w1/2 on the chromatrogram , find the number of plates, N1 and N2, and the plate height for these two compounds.

14.77 min
n-C,Hie
14.56 min
12.98 min
13.20 min
13.81 min
13.00
13.50
Time (min)
14.00
14.50

TABLE 23-2 Summary of chromatography equations
| Quantity
Partition coefficient
Equation
Parameters
K= cJc.
c, = concentration of solute in stationary phase
Cm = concentration of solute in mobile phase
1, = retention time of solute of interest
= retention time of unretained solute
4, = volume flow rate = volume per unit time
V, = volume of stationary phase
V = volume of mobile phase
1, = time solute spends in stationary phase = 1,- 1.
, = time solute spends in mobile phase
Adjusted retention time
Retention volume
Retention factor
V, =1, 4,
k = r.- KV/Vm
Relative retention (also
a =
Subscripts I and 2 refer to two solutes
called separation factor)
w = width at base
win = width at half-height
16
5.55
Number of plates
N=
win
Plate height
G = standard deviation of band
H =
x= distance traveled by center of band
L = length of column
N= number of plates on column
N
Ar,
AV.
Ar, = difference in retention times
AV, = difference in retention volumes
W = average width measured at baseline in same units
as numerator (time or volume)
Resolution
Resolution
VN (a - 1)
N= number of plates
a = relative retention
k = retention factor for second peak
Resolution =
(1 + k2

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