You perform a concentration of a single component using displacement chromatography, and the following data applies: Concentration of binding sites: 4*10^(-4) M Kd for displacer: 2.0*10^(-5) M Kd for component A: 4*10^(-5) M Kd for component B: 1.5*10^(-4) M The concentration of the injected displacer is

You perform a concentration of a single component using displacement chromatography, and the following data applies: Concentration of binding sites: 410^(-4) M Kd for displacer: 2.010^(-5) M Kd for component A: 410^(-5) M Kd for component B: 1.510^(-4) M The concentration of the injected displacer is

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Calculate the mass percent of the ascorbic acid in the same show full solution Situation: The pounded Vitamin C tablet was half into two below are the calculated data in the experiment. Each of the half are transfered in a 250ml erlen meyer flask and added 75ml of deionized water for the vitamin C to dissolve. Then it was prepared for titration where the burette was filled with 0.09794M standardize NaOH solution then a total of with 3 drops 2% phenolphthalein indicator was added to each erlen meyer flask l, swrill it and begin the titration. below are the following data gathered: Vitamins used: Nature Made vitamin C 500mg tablet Trial 1 Mass: 0.3108g of Vitamin C tablet Initial volume: 1.6mL Final volume: 15.4mL Trial 2 Mass: 0.3349g Initial volume: 15.4 mL Final volume: 30.2 mL
Chloroform is an internal standard in the determination of the pesticide DDT in a polarographic analysis in which each compound is reduced at an electrode surface. A mixture containing 0.500 mM chloroform and 0.800 mM DDT gave signals of 15.3 µA for chloroform and 10.1 µA for DDT. An unknown solution (10.00 mL) containing DDT was placed in a 100-ml volumetric flask and 10.2 ul of chloroform (MW = 119.39, density = 1.484 g/mL) were added. After dilution to the mark with solvent, polarographic signals of 29.4 and 8.7 µA were observed for the chloroform and DDT, respectively. Find the concentration of DDT in the unknown.
The figure below shows a typical separation of several fat-soluble vitamins by HPLC. The x-axis is retention time in minutes. From the graph try to estimate retention times for peaks 2 and 3. Retention time peak 2 = Retention time peak 3 = Assume that w1/2 is 0.15 minutes for peak 2 and 0.16 minutes for peak 3. Assume that tm is 2.00 minutes. How much time does gamma-tocopherol spend in the stationary phase? From tr and w1/2 find N for the peak that elutes first. From tr and w1/2 find the resolution of peaks 2 and 3 Calculate α for these peaks. If the flow rate for the column is 1.2 mL/minute what is the retention volume for peak 4?
A 200 mL water sample was treated with SPE column for pe- concentration of a certain pesticide. The final solution (24 mL) was analysed by HPLC and the .pesticide content was 1.2 ppm :The pre-concentration factor is 12.5 O 6.7 O 11.4 O 8.3
The results in the table below were obtained from the analyses of Mg2+ content in an orange juice sample using a flame-AAS. Based on these results answer the following questions: (i) (ii) Replicate 1 2 3 4 Mg²+(ppm) 7.08 7.21 7.12 7.09 Calculate the mean, standard deviation and coefficient of variation (or %RSD) for the results obtained. If the certified value of the Mg2+ content of the apple juice sample is 7.45 ppm. What is the percentage of recovery for this analysis?
The percentage purity of a paracetamol sample was determined by a reversed phase HPLC method as follows: • A calibration curve of peak area versus concentration (ug/mL) of pure paracetamol was constructed The paracetamol sample for analysis was prepared by taking 10.0254 g of powder and dissolving it in IL of distilled water (solution A). 1 mL of the latter solution was further diluted to 1L with distilled water (solution B). Solution B was analysed by HPLC analysis Results • The equation for the best-fit line of the calibration curve is: y = 26811x – 1313.8 The average peak area for the paracetamol sample (solution B) was 266796.2 Calculate the percentage purity of the paracetamol powder.
A solution contains 0.10 M Sr(NO3)₂ and 0.20 M Bi(NO₂). When solid Na AsO is added to the solution a precipitate forms. What concentration of AsO ³ maintains maximum separation of Sr²+ and Bi³+? Ksp of Sr₂(AsO₂)₂ = 4.29 x 10-1⁹ and BIASO = 4.43 x 10-1⁰ 4 4/2
PAPER CHROMATOGRAPHY Additional details: 20mL distilled water + 20mL 95% ethyl alcohol solution was used Main Question: Give the inferences (by color) of the following spots in the picture below
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