You perform a concentration of a single component using displacement chromatography, and the following data applies: Concentration of binding sites: 4*10^(-4) M Kd for displacer: 2.0*10^(-5) M Kd for component A: 4*10^(-5) M Kd for component B: 1.5*10^(-4) M The concentration of the injected displacer is

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You perform a concentration of a single
component using displacement
chromatography, and the following data
applies: Concentration of binding sites:
4*10^(-4) M Kd for displacer: 2.0*10^(-5) M Kd
for component A: 4*10^(-5) M Kd for
component B: 1.5*10^(-4) M The
concentration of the injected displacer is
2.0*10^(-4) M Determine and justify whether
the components are part of a displacement
system, and if so, provide their free and
bound concentrations. Assume that the
number of binding sites is the same for all
components.
Transcribed Image Text:You perform a concentration of a single component using displacement chromatography, and the following data applies: Concentration of binding sites: 4*10^(-4) M Kd for displacer: 2.0*10^(-5) M Kd for component A: 4*10^(-5) M Kd for component B: 1.5*10^(-4) M The concentration of the injected displacer is 2.0*10^(-4) M Determine and justify whether the components are part of a displacement system, and if so, provide their free and bound concentrations. Assume that the number of binding sites is the same for all components.
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