None Problem 2 (plate theory). Consider the scale-up of achromatography column for the purification of a protein. Acolumn of 40 cm length is used with a superficial velocity of 40cm/h. The peak elutes at 100 min. The standard deviation of thepeak is 14 min.(a) How long must you wait to collect 90% of the protein?(b) If the same column is used, but the velocity is increased to60 cm/hr and external or Taylor dispersion controls, whatwill be the value of σ.(c) If the column is lengthened to 60 cm while the velocity isat 60 cm/hr, how will σ change? Will the peak be sharperor broader than at 40 cm/hr with a 40 cm column?

Note that, Plate theory is a simplified model, and real chromatography behavior might deviate…

Answered: Problem 2 (plate theory). Consider the…

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.14QAP

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4.Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution time for unretained solute is 1.06 min. The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a layer of stationary phase 3.0 μm thick on the inner wall. a) Measruing the width, w, at the baseline on the chromatogram, find the number of plates for these two compounds b) Use your answer to (a) to find the resolution between the two peaks c) Using the number of plates N=sqrtN1*N2 with the values from (a) calcuate what the resolution should be and compare your answer with the measured resolution in b
Answer the questions by using the given graph below
A chromatographic analysis is performed with a sample containing toluene and benzene. The column length was 25 m and the flow rate 45 mL/min. Solute tR air 1.75 benzene (A) 7.00 1.85 toluene (B) 16.10 1.65 Calculate the theoretical plate height for each peak. Benzene: 109 mm/plate and Toluene 16 mm/plate Benzene: 105 mm/plate and Toluene 49.7 mm/plate Benzene: 172 mm/plate and Toluene 175 mm/plate Benzene: 119 mm/plate and Toluene 181 mm/plate
A volume of 250 ml of a 0.05 M solution of a reagent of formula weight (relative molecular mass) 40 was made up, the weighing being done by difference. The standard deviation of each weighing was 0.0001 g. 1 (a) What were the standard deviation and relative standard deviation of the weight of reagent used?
percentage purity of an ibuprofen sample was determined by a reversed phase HPLC method as follows: Y Der ● A calibration curve of peak area versus concentration (ug/mL) of pure ibuprofen was constructed The ibuprofen sample for analysis was prepared by taking 10.0204 g of powder and dissolving it in/1Lbf distilled water (solution A). 1 ml of the latter solution was further diluted to IL with distilled water (solution B). Solution B was analysed by HPLC analysis Results The equation for the best-fit line of the calibration curve is: y=26820x-1323.8. ● The average peak area for the ibuprofen sample (solution B) was 266796.2 Calculate the percentage purity of the ibuprofen powder.
6
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Consider the peaks for heptane (14.56 min) and p-difluorobenzene (14.77 min) in the chromatogram. The column is 30.0 m long. (a) Measure w1/2 for each peak and calculate the number of plates and the plate height for each. (b) From w1/2, compute w for each peak and find the resolution between the two peaks.
An analyst was measuring the performance of a HPLC column. The test solute was Toluene (5 µL). The column was a 4.6 mm i.d. column, 150 mm in length and packed with 5 µm C18 particles. The mobile phase was 40/60 water/methanol and the flow rate was 0.80 mL/min. The void time (to) was 1.86 minutes The retention time of the toluene sample was 14.50 min. The standard deviation of the peak was 7.0542 (s). 2a. The mobile phase velocity was? 0.0806 mm/s 0.1344 mm/s 1.344 mm/s 1.344 x 10³ mm/s (i) (ii) (iv) 2b. The number of theoretical plates was? (i) (ii) (iii) (iv) 2c. The height equivalent to a theoretical plate was? 1.041x10³ m 1.015x105 m 9.862x10-6 m 1.186x10¹² m (i) (ii) (iii) (iv) 4802 15210 24705 9848 2d. The reduced plate height was? (i) 2.03 (ii) 1.97 (iii) 7.55 (iv) 2.08 2e. The column was? (i) (ii) Extremely well packed Well packed Poorly packed (iii) (iv) Insufficient information to decide about the quality of the column
The results in the table below were obtained from the analyses of Mg2+ content in an orange juice sample using a flame-AAS. Based on these results answer the following questions: (i) (ii) Replicate 1 2 3 4 Mg²+(ppm) 7.08 7.21 7.12 7.09 Calculate the mean, standard deviation and coefficient of variation (or %RSD) for the results obtained. If the certified value of the Mg2+ content of the apple juice sample is 7.45 ppm. What is the percentage of recovery for this analysis?
Internal standard (As , Cs ), analyte with known concentration (Ax , Cx ) In a chromatographic equipment, a solution containing 0.006727 X and 0.008331 M S give peak area of Ax= 2316 and As= 207. To analyze an unknown sample, 5.0mL of 0.008331M S was added to 5.0mL of X, and the mixture was diluted to 50.0mL. This mixture gave a chromatography spectrum with area Ax= 3206 and As= 200. Find Cx.
Find the mean sand standard deviation of each sample.

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