A 20-cm long packed column was used to perform the separation of A and B by LC. TRA=16.28 min, TR,B=17.50 min, WA=1.11 min, WB=1.19 min, tм=1.30 min. (a) Calculate the retention factor (k') for A and B. (b) Calculate the average number of theoretical plates and plate height.

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A 20-cm long packed column was used to perform the separation of A and B by LC. TRA=16.28 min,
TR,B=17.50 min, WA=1.11 min, WB=1.19 min, tм=1.30 min.
(a) Calculate the retention factor (k') for A and B.
(b) Calculate the average number of theoretical plates and plate height.
Transcribed Image Text:A 20-cm long packed column was used to perform the separation of A and B by LC. TRA=16.28 min, TR,B=17.50 min, WA=1.11 min, WB=1.19 min, tм=1.30 min. (a) Calculate the retention factor (k') for A and B. (b) Calculate the average number of theoretical plates and plate height.
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