wo components in an HPLC separation have retention times that differ by 22 s. The first peak elutes in 10.5 min and the peak widths are approximately equal. Use a spreadsheet to find the minimum number of theoretical plates needed to achieve the following resolution, Rs, values: 0.50, 0.75, 0.90, 1.0, 1.10, 1.25, 1.50, 1.75, 2.0, and 2.5. How would the results change if peak 2 were twice as broad as peak 1?

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Q: Substances A and B have retention times of 12.75 and 14.28 min, respectively, on a 30.0-cm column.…

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Q: Consider a separation performed on a 50.0 mm long open tubular column with a 0.50 mm diameter and a…

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Q: A mixture of A and B is injected into a liquid chromatographic column with length of 45 cm. The…

A: given- length of the plate= L = 45 cm plate height = H = ?

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Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.