Bradford assay was used to determine bovine serum albumin (BSA) concentrations. The method works by binding Coomassie Brilliant Blue dye to protein standards and unknown, leading to a shift in the absorbance maximum of the dye. A stock solution of BSA is available (10 mg/ml) from which the protein standards were prepared. Bradford reagent, 290 ul, was pipetted into a transparent 96-well microplate. 10 ul of the protein dilution was added followed by mixing in the wells. After 5 min of incubation at room temperature, the plate was read in spectrum mode with the absorbance spectrometer of the microplate reader. The spectrum of the dye not bound to protein was also determined. Create a standard curve or regression equation that related the protein concentration and absorbance. An unknown BSA sample was prepared the same way as the standard and the OD was 0.35. determine the original concentration of the protein solution. The measurements can be seen below. Protein Concentration Average Absorbance 0.0625 0.02 0.125 0.03 0.25 0.05 0.5 0.09 1 0.22

Bradford assay was used to determine bovine serum albumin (BSA) concentrations. The method works by binding Coomassie Brilliant Blue dye to protein standards and unknown, leading to a shift in the absorbance maximum of the dye. A stock solution of BSA is available (10 mg/ml) from which the protein standards were prepared. Bradford reagent, 290 ul, was pipetted into a transparent 96-well microplate. 10 ul of the protein dilution was added followed by mixing in the wells. After 5 min of incubation at room temperature, the plate was read in spectrum mode with the absorbance spectrometer of the microplate reader. The spectrum of the dye not bound to protein was also determined. Create a standard curve or regression equation that related the protein concentration and absorbance. An unknown BSA sample was prepared the same way as the standard and the OD was 0.35. determine the original concentration of the protein solution. The measurements can be seen below. Protein Concentration Average Absorbance 0.0625 0.02 0.125 0.03 0.25 0.05 0.5 0.09 1 0.22

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

A gold on iron oxide catalyst was prepared by wet impregnation and dried at 110 °C overnight. The sample was then subjected to XPS analysis, and the following peak areas determined from the high resolution spectra: Integrated Peak Area Element Sensitivity Factor C 13587 0.278 5187 0.78 Fe 13587 2.957 Au 4000 6.25
Anionic surfactants in wastewater are measured spectrophotometrically as methylene blue active substances (MBAS). A volume of a water sample is first reacted with methylene blue, a cationic dye, in a basic buffered aqueous solution. The resulting solution is agitated after the addition of chloroform. The organic layer is then extracted, and the absorbance is read at 655 nm. A standard calibration curve is produced by plotting absorbance vs. concentration for the concentration range between 0 ppm to 2.50 ppm. The line had the following equation: y = 0.286x + 0.009 a. The limit of surfactant content in Philippine class B water is 300 ppb. If a 5 mL aliquot of a 50 mL water sample had an absorbance of 0.084 after MBAS analysis, is the sampled water body suitable for bathing? Support your answer
using the following information to construct a valid hypothesis addressing the effect of pH and temperature on the hydrolysis of starch. Per bench, prepare the stock amylase enzyme solution by adding distilled water to the powdered enzyme in the flask , each bench will share one flaks of enzyme solution. per groups, turn on the spectrometer and set the wavelength to 560nm.allow the instruments to warm up for at least 10 minutes. per group, set up 17 cuvettes in a Styrofoam rack. Add 3 drops of iodine (IKI) to each coveter. per group, prepare the “black” cuvette by rapidly adding 3 mL of maltose standard solution to one of The prepared cuvettes containing IKI. Rapid addition of the maltose will mix the maltose and IKI
Which window size gives you a better signal-to-noise ratio? 7-residue window 15-residue window Under what circumstances would it be important to use a narrower window? Smaller range windows alter the sensitivity of the analysis, increasing the signal-to-noise ratio. Narrow windows permit identification of membrane-spanning regions at either end of the protein. Narrow windows allow hydropathy analysis of very small peptides that have less than 10 amino acid residue Smaller window ranges identify hydrophilic regions of the protein that are likely surface-exposed.
Aa.53.
The concentration of butanol 1.5M. Calculate the molar absorptivity ? when the solution produced an absorbance of 3.2 when using a spectrophotometer with a cuvette path length of l = 1.0 cm
12:15 9 & h b • 3r ill %19 Belge (1) - Kaydedildi İngilizce (ABD) dilindeki metin denetlenmiyor. .. A researcher prepared mixed solutions at different concentrations and measured the absorbances with the help of a spectrophotometer. In the spectrophotometer device, the distance between the cuvette and the light diffuser is 3 cm. The absorbance values of the mixture given in the table below. Mixture V (ml) %T Absorbans Concentrations (g/l) 5 37,55 0,444 0,21M 40,89 0,372 0,26M 3 44,97 0,311 0,35M 2 67,50 0,172 0,71M 1 82,65 0,084 0,97M a) Explain how to prepare 1N H2504 solution. b) Calculate the e values and the total absorbance for each volume value opposite. c) Explain the relationship between permeability and absorbance. KT A e A E E II ...
Kindly answer this question i, ii & iii
A student mixed 5.00 mL of 0.100 M Fe(NO3)3 with 50.00 mL of 2.00x10−4 M KSCN. The student measured the absorbance of one aliquot of the solution at a wavelength of 447 nm, with a pathlength of 1.00 cm. The absorbance reading was 0.615. Compute the following quantities a. [FeSCN2+]eq = b. (mols FeSCN2+)eq = c. (mols Fe3+)initial = d. (mols SCN−)initial = e. (mols Fe3+)eq = f. (mols SCN−)eq = g. [Fe3+]eq = h. [SCN−]eq = i. Keq=
An atomic absorption method for the determination of the amount of iron present in used jet engine oil was found from pooling 30 triplicate analyses to have a standard deviation s = 3.1 µg Fe/mL. If s is a good estimate of o, calculate the 95 and 99% confidence intervals for the result 21.7 µg Fe/mL if it was based on the following criteria. (a) a single analysis 95% confidence interval= 21.7 + 99% confidence interval = 21.7 + (b) the mean of two analyses 95% confidence interval = 21.7 + 99% confidence interval= (c) the mean of six analyses 21.7 ± 95% confidence interval = 21.7 + 99% confidence interval= 21.7 + μg Fe/mL μg Fe/mL μg Fe/mL μg Fe/mL μg Fe/mL μg Fe/mL
1
SPECTROPHOTOMETRY Two groups in a BIO 120 lab extracted a blue-violet pigment from mayana plant which was to be used as a natural dye for Philippine textiles. To determine the concentration of the pigment isolated, each group made a standard curve using commercially available indigo dye in dimethylsulfoxide (DMSO). The following data were obtained by the two groups: Table 4. Absorbance readings of standard solutions of varying concentrations of indigo dye in DMSO. Pure DMSO 0.115 Standard solutions (ppm) Group 1 Data Group 2 Data 0.2 0.398 0.224 0.4 0.563 0.437 0.6 0.732 0.603 0.8 0.833 0.811 1.0 1.008 0.966 1. Show the constructed standard curves of the two groups with proper figure titles. 2. Which of the two groups generated a better standard curve? Justify your answer by providing the R? value for each data set. 3. The extracted pigment has an absorbance of 0.983. When a 0.2 mL-aliquot of the extract was diluted to 1.0 mL, the absorbance of the diluted solution was found to be…