(a) Use the information given below to sketch and label a molecular exclusion calibration curve. Use the sketch to explain how the polymer standards in the sample would migrate through the size exclusion column. Information for Q2 (a): A gel permeation chromatographic column contains a 104 Å stationary phase, which has a relative molecular mass range of 5 x 103 – 6 x 105 Daltons. Four polymer standards, with relative molecular masses of 8 x 105, 4 x 105, 7 x 103, or 4 x 103 Daltons, are dissolved in benzene and injected into the chromatograph. The void volume of the column is 2 cm3, the interstitial volume is 8 cm3.

(a) Use the information given below to sketch and label a molecular exclusion calibration curve. Use the sketch to explain how the polymer standards in the sample would migrate through the size exclusion column. Information for Q2 (a): A gel permeation chromatographic column contains a 104 Å stationary phase, which has a relative molecular mass range of 5 x 103 – 6 x 105 Daltons. Four polymer standards, with relative molecular masses of 8 x 105, 4 x 105, 7 x 103, or 4 x 103 Daltons, are dissolved in benzene and injected into the chromatograph. The void volume of the column is 2 cm3, the interstitial volume is 8 cm3.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

Write the missing chemical formula or name for each of the following substances listed in the procedure.
5
As you investigate the relationship of oil and plastic, add “zoom out boxes” to the images below. Each “zoom-out box” should provide a deep explanation of the relationship between oil, water bottles, and society. ( Use the images from the model )
Can i get help please
please help me
Carbon dioxide concentration (parts per million) Examine the graph and answer the following questions. Part A Atmospheric Carbon Dioxide What was the average rate of increase in carbon doxide concentration between 1900 and 19407 410 400 Express you answer in parts per million per year to two significant figures. 300 380 a ? 370 360 ppm/year 350 340 Submit Bequest Anawer 330 320 310 Part 8 Complete previous parts) 300 290 - 1860 1880 1900 1920 1940 1960 1980 2000 2020 Provide Feedback Next> Year
Can i get step by step help please
help me
Describe the Integrated Law?
Can i get help please
Land uses like using plants to produce bioplastics- does it really help the environment in the long run?
1. Explain the “energy ladder” (moving up and down) 2. Explain how this affects pollution/disorder.