Answered: You have carried out an experiment…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

You have carried out an experiment using the spectrophotometry concept. A solution
containing compound X is mixed with reagent 1 and then reagent 2. This mixture produces a
blue colour whose absorbance (A) could be read at 550 nm. The results are shown below

If the standard solution (compound X) used have a concentration of 1 mM:

3. If the absorbance was read using 1 cm diameter cuvettes, calculate the molar extinction
coefficient for compound X in the solution. State the unit for the coefficient. Assume
each molecule of X produces one molecule of coloured compound

Test
tube no.
2
3
4
5
6
7
Sample
Y
Standard
solution X
(ml)
0
0.1
0.2
0.4
0.6
0.8
1.0
1.0
Reagent 1 Reagent 2
(ml)
(ml)
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
Water (ml)
1.0
0.9
0.8
0.6
0.4
0.2
0.0
0.0
A550 nm
0.0
0.05
0.12
0.24
0.35
0.48
0.59
0.35

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