For determining the %w/w of salicylamide and caffeine in an unknown sample using acetaminophen as an internal standard using a HPLC a) Is it possible to use the λmax (273 nm) of caffeine in the experiment instead of using 254 nm? Explain your reasoning. b) What are some advantages of using the Internal
For determining the %w/w of salicylamide and caffeine in an unknown sample using acetaminophen as an internal standard using a HPLC a) Is it possible to use the λmax (273 nm) of caffeine in the experiment instead of using 254 nm? Explain your reasoning. b) What are some advantages of using the Internal
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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1. For determining the %w/w of salicylamide and caffeine in an unknown sample using acetaminophen as an internal standard using a HPLC
a) Is it possible to use the λmax (273 nm) of caffeine in the experiment instead of using 254 nm? Explain your reasoning.
b) What are some advantages of using the Internal Standard method for quantitation over the External Standard method?
c) How is the signal intensity seen in the chromatogram affected by the wavelength used for analysis?
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