For determining the %w/w of salicylamide and caffeine in an unknown sample using acetaminophen as an internal standard using a HPLC a) Is it possible to use the λmax (273 nm) of caffeine in the experiment instead of using 254 nm? Explain your reasoning. b) What are some advantages of using the Internal
For determining the %w/w of salicylamide and caffeine in an unknown sample using acetaminophen as an internal standard using a HPLC a) Is it possible to use the λmax (273 nm) of caffeine in the experiment instead of using 254 nm? Explain your reasoning. b) What are some advantages of using the Internal
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter20: Molecular Mass Spectrometry
Section: Chapter Questions
Problem 20.16QAP
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1. For determining the %w/w of salicylamide and caffeine in an unknown sample using acetaminophen as an internal standard using a HPLC
a) Is it possible to use the λmax (273 nm) of caffeine in the experiment instead of using 254 nm? Explain your reasoning.
b) What are some advantages of using the Internal Standard method for quantitation over the External Standard method?
c) How is the signal intensity seen in the chromatogram affected by the wavelength used for analysis?
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