The levels of Vitamin B1 in a sample of milk was determined using the Standard Addition method and technique of fluorescence spectroscopy. 18.0 ml of the milk sample was diluted to 20.0 ml using distilled water and then the fluorescence was measured. The resulting signal was 210 units. A spike was made by taking 18.0 ml of the same milk sample, adding 1.0 ml of a 6.0 ppm Vitamin B1 standard solution, and then diluting to 20.0 ml using distilled water. The signal of the spiked milk was 540 units. Calculate the concentration of Vitamin B1 in the original milk sample (in ppm). Report to 3 decimal places.

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The levels of Vitamin B1 in a sample of milk was determined using the Standard
Addition method and technique of fluorescence spectroscopy.
18.0 ml of the milk sample was diluted to 20.0 ml using distilled water and then the
fluorescence was measured. The resulting signal was 210 units.
A spike was made by taking 18.0 ml of the same milk sample, adding 1.0 ml of a 6.0
ppm Vitamin B1 standard solution, and then diluting to 20.0 ml using distilled
water. The signal of the spiked milk was 540 units.
Calculate the concentration of Vitamin B1 in the original milk sample (in ppm).
Report to 3 decimal places.
Transcribed Image Text:The levels of Vitamin B1 in a sample of milk was determined using the Standard Addition method and technique of fluorescence spectroscopy. 18.0 ml of the milk sample was diluted to 20.0 ml using distilled water and then the fluorescence was measured. The resulting signal was 210 units. A spike was made by taking 18.0 ml of the same milk sample, adding 1.0 ml of a 6.0 ppm Vitamin B1 standard solution, and then diluting to 20.0 ml using distilled water. The signal of the spiked milk was 540 units. Calculate the concentration of Vitamin B1 in the original milk sample (in ppm). Report to 3 decimal places.
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