The chromatogram in the figure is of a mixture of 2 compounds, carried out in a packed liquid chromatographic column of 25 cm length. The flow rate was 0.40 mL min-¹. A species that is not retained passes through the column in 5 minutes. a) Determine the retention factors for the two components. b) Calculate the resolution of the two peaks. c) Find the average height of the plate. d) What column length would be needed to achieve a resolution of 1.75? Detector response 0 5.0 A 22.0 20 30.0 30 Time, min 38.0 40 39.0 50 50.0 60 62.0 70

The chromatogram in the figure is of a mixture of 2 compounds, carried out in a packed liquid chromatographic column of 25 cm length. The flow rate was 0.40 mL min-¹. A species that is not retained passes through the column in 5 minutes. a) Determine the retention factors for the two components. b) Calculate the resolution of the two peaks. c) Find the average height of the plate. d) What column length would be needed to achieve a resolution of 1.75? Detector response 0 5.0 A 22.0 20 30.0 30 Time, min 38.0 40 39.0 50 50.0 60 62.0 70

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

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The chromatogram in the figure is of a mixture of 2 compounds,
carried out in a packed liquid chromatographic column of 25 cm
length. The flow rate was 0.40 mL min ¹. A species that is not retained
passes through the column in 5 minutes.
a) Determine the retention factors for the two components.
b) Calculate the resolution of the two peaks.
c) Find the average height of the plate.
d) What column length would be needed to achieve a resolution of
1.75?
Detector response
0
5.0
10
22.0
20
30.0
30
Time, min
38.0
40
39.0
50
50.0
60
62.0
70

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Step 2: a) Calculation of retention factors

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Step 3: b) Calculation of resolution

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Step 4: c) Calculation of Average height of the plate

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