An open tubular chromatography column is 28.7 m long with an inner diameter of 0.503 mm . The inside wall is coated with a layer of 3.30 µm thick stationary phase. Unretained solute passes through the column in 3.45 min (tM , and a certain solute has a retention time of 16.75 min (†R). What is the linear flow rate? m linear flow rate: min min What is the volume flow rate? mL volume flow rate: min Calculate the retention factor, k, for the solute. k = Calculate the distribution constant, Kp. Kp= What fraction of time did the solute spend in the stationary phase? fraction of time in the stationary phase:

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