3 questions... [2] How might a better chromatographic separation of solutes be accomplished?[3] What would happen if a pen was used to mark the starting line and ion application points?[4] Why is it necessary to keep the point of application small? How does the size of the applied samplearea affect the chromatographic separation and the Rf value?

Answered: [2] How might a better chromatographic…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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3 questions...

[2] How might a better chromatographic separation of solutes be accomplished?
[3] What would happen if a pen was used to mark the starting line and ion application points?
[4] Why is it necessary to keep the point of application small? How does the size of the applied sample
area affect the chromatographic separation and the Rf value?

Expert Solution

Step 1

Chromatography is one of the most important techniques that is used analytical techniques. It is used as method to analyze the components in the mixture as well as separate them.

There are different types of chromatographic techniques such as column chromatography, thin layer chromatography, paper chromatography, etc. However, the principle of these techniques remain the same. Chromatography as mobile phase and stationary phase. Different components have different level of affinity towards both the phases. Therefore, components are separated based on this different in affinity towards each phase.