Below are some real data I collected doing a glutamate uptake experiment. I plated astrocytes in a dish and applied glutamate spiked with a radioactive tracer. Because I was measuring glutamate transport (and not binding of glutamate to a receptor), I measured velocity instead of binding: pmoles of glutamate transported per minute, normalized to the amount of protein (mg) in the cells. Use the figure to answer the following questions: Velocity/Concentration (pmol/mg/min/uM) 300 250 200 150 100 50 Glutamate Uptake Pharmacodynamics y = -0.0158x + 119.79 y = -0.0169x + 271.33 0 0 2000 4000 6000 8000 10000 12000 14000 16000 18000 Velocity (pmol/mg/min) Control Condition Experimental Condition

Below are some real data I collected doing a glutamate uptake experiment. I plated astrocytes in a dish and applied glutamate spiked with a radioactive tracer. Because I was measuring glutamate transport (and not binding of glutamate to a receptor), I measured velocity instead of binding: pmoles of glutamate transported per minute, normalized to the amount of protein (mg) in the cells. Use the figure to answer the following questions: Velocity/Concentration (pmol/mg/min/uM) 300 250 200 150 100 50 Glutamate Uptake Pharmacodynamics y = -0.0158x + 119.79 y = -0.0169x + 271.33 0 0 2000 4000 6000 8000 10000 12000 14000 16000 18000 Velocity (pmol/mg/min) Control Condition Experimental Condition

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 25SST

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One of the important uses of the Nernst equation is in describing the flow of ions across plasma membranes. Ions move under the influence of two forces: the concentration gradient (given in electrical units by the Nernst equation) and the electrical gradient (given by the membrane voltage). This is summarized by Ohms law: Ix=Gx(VmEx) which describes the movement of ion x across the membrane. I is the current in amperes (A); G is the conductance, a measure of the permeability of x, in Siemens (S), which is I/V;Vm is the membrane voltage; and Ex is the equilibrium potential of ion x. Not only does this equation tell how large the current is, but it also tells what direction the current is flowing. By convention, a negative value of the current represents either a positive ion entering the cell or a negative ion leaving the cell. The opposite is true of a positive value of the current. a. Using the following information, calculate the magnitude of Na [ Na+ ]0=145mM,[ Na+ ]i=15mM,Gna+=1nS,Vm=70mV b. Is Na+ entering or leaving the cell? c. Is Na+ moving with or against the concentration gradient? Is it moving with or against the electrical gradient?
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