DNA polymerase occasionally incorporates the wrong nucleotide during DNA replication. If left unrepaired, the base-pair mismatch that results will lead to mutation in the next replication. As part of a template strand, the incorporated wrong base will direct the incorporation of a base complementary to itself, so the bases on both strands of the DNA at that position will now be different from what they were before the mismatch event. The MM-minus strain of yeast does not have a functional mismatch excision repair system, but it has normal base excision repair and nucleotide excision repair systems. Which of the following statements is correct about differences in the mutation spectrum between MMR-minus and wildtype yeast? More than one answer is correct. (a) More point mutations will arise in MMR-minus yeast. Fewer point mutations will arise in MMR-minus yeast as compared with wildtype. (b) Of the total point mutations that do occur, the fraction in which G is replaced by C will be lower in MMR-minus yeast than in wildtype. (Hint: imagine the very first mismatch event and ask yourself if base excision repair could handle it.) (d) Of the total point mutations that do occur, the fraction of in which G is replaced by A will be lower in MMR-minus yeast than in wildtype. (e) Of the total point mutations that do occur, the fraction in which A is replaced by G will be lower in MMR-minus yeast.
DNA polymerase occasionally incorporates the wrong nucleotide during DNA replication. If left unrepaired, the base-pair mismatch that results will lead to mutation in the next replication. As part of a template strand, the incorporated wrong base will direct the incorporation of a base complementary to itself, so the bases on both strands of the DNA at that position will now be different from what they were before the mismatch event. The MM-minus strain of yeast does not have a functional mismatch excision repair system, but it has normal base excision repair and nucleotide excision repair systems. Which of the following statements is correct about differences in the mutation spectrum between MMR-minus and wildtype yeast? More than one answer is correct. (a) More point mutations will arise in MMR-minus yeast. Fewer point mutations will arise in MMR-minus yeast as compared with wildtype. (b) Of the total point mutations that do occur, the fraction in which G is replaced by C will be lower in MMR-minus yeast than in wildtype. (Hint: imagine the very first mismatch event and ask yourself if base excision repair could handle it.) (d) Of the total point mutations that do occur, the fraction of in which G is replaced by A will be lower in MMR-minus yeast than in wildtype. (e) Of the total point mutations that do occur, the fraction in which A is replaced by G will be lower in MMR-minus yeast.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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Question
DNA polymerase occasionally incorporates the wrong nucleotide during DNA
replication. If left unrepaired, the base-pair mismatch that results will lead to
mutation in the next replication. As part of a template strand, the incorporated
wrong base will direct the incorporation of a base complementary to itself, so the
bases on both strands of the DNA at that position will now be different from what
they were before the mismatch event.
The MM-minus strain of yeast does not have a functional mismatch excision repair
system, but it has normal base excision repair and nucleotide excision repair systems.
Which of the following statements is correct about differences in the mutation
spectrum between MMR-minus and wildtype yeast?
More than one answer is correct.
(a) More point mutations will arise in MMR-minus yeast.
Fewer point mutations will arise in MMR-minus yeast as compared with
wildtype.
(b) Of the total point mutations that do occur, the fraction in which G is replaced by
C will be lower in MMR-minus yeast than in wildtype. (Hint: imagine the very
first mismatch event and ask yourself if base excision repair could handle it.)
(d) Of the total point mutations that do occur, the fraction of in which G is replaced
by A will be lower in MMR-minus yeast than in wildtype.
(e) Of the total point mutations that do occur, the fraction in which A is replaced by
G will be lower in MMR-minus yeast.
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