Aliens with orang eye color allele (o) is recessive to the dominant black eye color allele (O). The locus of the orange gene from 10 pure breed orange eyes aliens and 10 pure breed black eyes aliens. You notice a difference in the DNA sequence linked to each allele of the orange gene and you decide to use it as a physical marker to follow recombination between this sequence linked to the orange gene. These sequences consist of short tandem repeat (STR) with two different number of repeats, each associated with one of the two orange gene alleles. a PCR test distinguishes the 10 repeat STR and the 6 repeat STR associated with the O and o alleles respectively. Using primers on each side of the STRs you can amplify by PCR this sequence and visualizing the size of the 10 repeat and 6 repeat STRs in an electrophoresis gel. You can follow the two STR sequences (10 and 6 repeats) linkage and recombination frequencies with the orange gene locus. The black eye aliens yield a PCR fragment that is longer STR (10 repeats) if they are homozygous or two PCR fragments (10 and 6 repeats) if they are heterozygous, whereas orange eyes only yield the 6 repeat STR PCR fragment. These repeats are only 4 cM apart from the orange gene locus. Design a pedigree that would have revealed which orange gene allele was dominant and which allele was recessive.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Aliens with orang eye color allele (o) is recessive to the dominant black eye color allele (O). The locus of the orange gene from 10 pure breed orange eyes aliens and 10 pure breed black eyes aliens. You notice a difference in the DNA sequence linked to each allele of the orange gene and you decide to use it as a physical marker to follow recombination between this sequence linked to the orange gene. These sequences consist of short tandem repeat (STR) with two different number of repeats, each associated with one of the two orange gene alleles. a PCR test distinguishes the 10 repeat STR and the 6 repeat STR associated with the O and o alleles respectively. Using primers on each side of the STRs you can amplify by PCR this sequence and visualizing the size of the 10 repeat and 6 repeat STRs in an electrophoresis gel. You can follow the two STR sequences (10 and 6 repeats) linkage and recombination frequencies with the orange gene locus. The black eye aliens yield a PCR fragment that is longer STR (10 repeats) if they are homozygous or two PCR fragments (10 and 6 repeats) if they are heterozygous, whereas orange eyes only yield the 6 repeat STR PCR fragment. These repeats are only 4 cM apart from the orange gene locus. Design a pedigree that would have revealed which orange gene allele was dominant and which allele was recessive.

a) O (black) allele is dominant to the o (orange) allele
Black eyes
(0/0 or Olo)
c) What are the possible parental and recombinant children
from this marriage
(10)
O
Orange eyes
(o/o)
X
(6)
offere
b) DNA marker locus with STR alleles
Short Tandem Repeat (STR) alleles
STR allele with
Left PCR 6 repeats
primer
e
One repeat
Right PCR
primer
STR allele with
10 repeats
1
PCR, gel electrophoresis
STR genotype
(6,6) (10,10) (6,10)
Phenotype:
DNA bands
seen after gel
electrophoresis

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