Transgenic tobacco plants were obtained in which the vector Ti plasmid was designed to insert the gene of interest plus an adjacent kanamycin-resistance gene. The inheritance of chromosomal insertion was followed by testing progeny for kanamycin resistance. Two plants typified the results obtained generally. When plant 1 was backcrossed with wild-type tobacco, 50 percent of the progeny were kanamycin resistant and 50 percent were sensitive. When plant 2 was backcrossed with the wild type, 75 percent of the progeny were kanamycin resistant and 25 percent were sensitive. What must have been the difference between the two transgenic plants? What would you predict about the situation regarding the gene of interest?
Transgenic tobacco plants were obtained in which the
vector Ti plasmid was designed to insert the gene of
interest plus an adjacent kanamycin-resistance gene.
The inheritance of chromosomal insertion was followed by testing progeny for kanamycin resistance.
Two plants typified the results obtained generally.
When plant 1 was backcrossed with wild-type tobacco,
50 percent of the progeny were kanamycin resistant
and 50 percent were sensitive. When plant 2 was backcrossed with the wild type, 75 percent of the progeny
were kanamycin resistant and 25 percent were sensitive. What must have been the difference between the
two transgenic plants? What would you predict about
the situation regarding the gene of interest?
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