R. Alu 100 100 300 F ----- 100 100 Where would you expect to see a band or bands if you used PCR to amplified this region - using the primers indicated above and DNA from an individual that was heterozygous - and loaded that PCR sample into the gel below?
R. Alu 100 100 300 F ----- 100 100 Where would you expect to see a band or bands if you used PCR to amplified this region - using the primers indicated above and DNA from an individual that was heterozygous - and loaded that PCR sample into the gel below?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question

Transcribed Image Text:HELP
800 bp
700 bp
600 bp
500 bp
400 bp
300 bp
200 bp
100 bp
Select all that apply:
a. At 100 bp
cross out
b. At 200 bp
cross out
c. At 300 bp
cross out
d. At 400 bp
cross out
cross out
e.
At 500 bp
cross out
f.
At 600 bp
cross out
g. At 700 bp
cross out
h. At 800 bp
cross out
At 900 bp
cross out
J.
At 1000 bp

Transcribed Image Text:The image below shows two variations of the same region on a chromosome. The top variant has an Alu element inserted into the middle of
the region where as the bottom variant has no insert. Arrows above the chromosome indicate the binding position of the the forward (F) and
reverse (R) primers. The numbers below each chromosome indicate distances, in base pairs (bp), of the relevant features.
F
Alu
100
100
300
-----
100
100
Where would you expect to see a band or bands if you used PCR to amplified this region - using the primers indicated above and DNA from an
individual that was heterozygous - and loaded that PCR sample into the gel below?
DNA
Ladder Sample
1000 bp
900 bp
800 bp
700 bp
600 bp
500 bp
400 bp
300 bp
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