R. Alu 100 100 300 F ----- 100 100 Where would you expect to see a band or bands if you used PCR to amplified this region - using the primers indicated above and DNA from an individual that was heterozygous - and loaded that PCR sample into the gel below?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question
HELP
800 bp
700 bp
600 bp
500 bp
400 bp
300 bp
200 bp
100 bp
Select all that apply:
a. At 100 bp
cross out
b. At 200 bp
cross out
c. At 300 bp
cross out
d. At 400 bp
cross out
cross out
e.
At 500 bp
cross out
f.
At 600 bp
cross out
g. At 700 bp
cross out
h. At 800 bp
cross out
At 900 bp
cross out
J.
At 1000 bp
Transcribed Image Text:HELP 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp Select all that apply: a. At 100 bp cross out b. At 200 bp cross out c. At 300 bp cross out d. At 400 bp cross out cross out e. At 500 bp cross out f. At 600 bp cross out g. At 700 bp cross out h. At 800 bp cross out At 900 bp cross out J. At 1000 bp
The image below shows two variations of the same region on a chromosome. The top variant has an Alu element inserted into the middle of
the region where as the bottom variant has no insert. Arrows above the chromosome indicate the binding position of the the forward (F) and
reverse (R) primers. The numbers below each chromosome indicate distances, in base pairs (bp), of the relevant features.
F
Alu
100
100
300
-----
100
100
Where would you expect to see a band or bands if you used PCR to amplified this region - using the primers indicated above and DNA from an
individual that was heterozygous - and loaded that PCR sample into the gel below?
DNA
Ladder Sample
1000 bp
900 bp
800 bp
700 bp
600 bp
500 bp
400 bp
300 bp
Transcribed Image Text:The image below shows two variations of the same region on a chromosome. The top variant has an Alu element inserted into the middle of the region where as the bottom variant has no insert. Arrows above the chromosome indicate the binding position of the the forward (F) and reverse (R) primers. The numbers below each chromosome indicate distances, in base pairs (bp), of the relevant features. F Alu 100 100 300 ----- 100 100 Where would you expect to see a band or bands if you used PCR to amplified this region - using the primers indicated above and DNA from an individual that was heterozygous - and loaded that PCR sample into the gel below? DNA Ladder Sample 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps with 1 images

Blurred answer
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON