Suppose that you could inject a wild-type mouse zygote with a specific CRISPR RNA and the Cas9 enzyme. The RNA directs the Cas9 enzyme to make adouble-strand break within a gene that you think maybe responsible for a heritable disease. Diagram inrough form how you might inject at the same time another nucleic acid molecule (here, a double-strandedDNA) to exploit homologous recombination so thatyou could convert the wild-type allele of the gene to aspecific mutant allele.
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
Suppose that you could inject a wild-type mouse zygote with a specific CRISPR RNA and the Cas9 enzyme. The RNA directs the Cas9 enzyme to make a
double-strand break within a gene that you think may
be responsible for a heritable disease. Diagram in
rough form how you might inject at the same time another
DNA) to exploit homologous recombination so that
you could convert the wild-type allele of the gene to a
specific mutant allele.
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