Abands). Please, àdestity he 10. Samples were tested for sickle cell disease by PCR. The results were analyzed by agarose gel clectrophoresis. Lanes I, 3, 5, 7, and 9 are PCR-amplificd, but undigested DNA (364 bp), and lanes 2, 4, 6, 8, and 10 are Ddel-digested DNA. Lanes I&2 are from a known carrier DNA genotype in lancs 3&4, 5&6, 7&8, 9&10. (B/B*, 4 bands). Please, identify the DNA marker 1 2 3 4 5 6 7 8 9 10 euene 364 bp- 291 bp- 201 bp- 90 bp- CKO BA/ßs

Abands). Please, àdestity he 10. Samples were tested for sickle cell disease by PCR. The results were analyzed by agarose gel clectrophoresis. Lanes I, 3, 5, 7, and 9 are PCR-amplificd, but undigested DNA (364 bp), and lanes 2, 4, 6, 8, and 10 are Ddel-digested DNA. Lanes I&2 are from a known carrier DNA genotype in lancs 3&4, 5&6, 7&8, 9&10. (B/B*, 4 bands). Please, identify the DNA marker 1 2 3 4 5 6 7 8 9 10 euene 364 bp- 291 bp- 201 bp- 90 bp- CKO BA/ßs

Biology: The Unity and Diversity of Life (MindTap Course List)

15th Edition

ISBN:9781337408332

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter15: Studying And Manipulating Genomes

Section: Chapter Questions

Problem 16SQ: Match the method with the appropriate enzyme. _____ PCR a. Taq polymerase _____ cutting DNA b. DNA...

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Match the method with the appropriate enzyme. _____ PCR a. Taq polymerase _____ cutting DNA b. DNA ligase _____ cDNA synthesis c. reverse transcriptase _____ DNA sequencing d. restriction enzyme _____ pasting DNA e. DNA polymerase (not Taq)
1. An image of a DNA profile is shown below. The size marker fragments are not shown, but the size in bp is displayed along the top. 100 150 200 250 300 350 D8 Identifiler TM k 2000 multiplex STR TH01 1500 D13 D19 D5 VWA ТРОХ D16 CSF AMEL D3 D21 FGA 1000 D18 D7 D2 500 How do the multiplex STR data demonstrate the profile is from a man? Which loci are homozygous? How do you know this? How does the IdentifierTM kit differentiate D19 and D3 PCR products? How does the IdentifierTM kit differentiate D5 and FGA PCR products?
1) you have a locus in your PCR reaction that is a DNA squence is on the fifth chromosome, is a single copy locus, and has a squcne ID# 818. What is a name according to ISFG nomenclature 2) in electrophoresis system, DNA migartes toward____ which is _____ charged.
4. Animation 12.4: This table shows results from a genetic test conducted on DNA samples from two patients. The patients are a newly married couple seeking genetic counseling about their risks of passing on a genetic disease to any children they may have in the future. The samples included the DNA region including and immediately surrounding a single gene. Restriction enzyme cleavage analyses were performed on the samples. Female Patient 1) 3400 bp fragment Male Patient 1) 1600 bp fragment 2) 1800 bp fragment 3) 3400 bp fragment DNA fragments on gel What do the genetic test results indicate about this case? O Both the woman and the man are homozygous for the mutation. O Both the woman and the man are heterozygous. O The woman is homozygous for the mutation and the man is homozygous for the normal gene. O The woman is homozygous for the mutation and the man is heterozygous.
2) A scientist wants to increase the amount of sample of a DNA sequence of 100 bps. Calculate the number cycles of PCR required to generate 1 ng of DNA, when starting from a single copy. Note that the molar mass of an average DNA bp is 600 g/mol.
Worksheet for Lab 12: DNA FINGERPRINTING Name Lab Day & Time - Negative End Marker Crime Suspect Suspect Standard Scene # 1 # 2 wells to eadms d al ib mot + Positive End nian AMa hos yb 1. When you simply look at the banding patterns on the gel from the DNA fingerprint we ran in lab as seen above, which two profiles match? 2. Does the fact that one suspect's DNA fingerprint matches the DNA at the crime scene prove their guilt? Why or why not? 3. The DNA was originally placed in the wells of the gel near the negative end of the gel and fragments moved towards the positive end of the gel. What charge do DNA molecules have? Positive 4a. Where on the gel are the longest fragments found? de gel, on lectrode 4b. Where on the gel are the shortest fragments found? IX che samples is mixeu w the sample to sink to the bottom of the wen. ally followed as it moves down the gel. TE buffer is matched to the gef TO assure- - bottom of the wells with the micropipette tip. tips contaminated with DNA in…
5. Show the separation pattern of the following DNA molecules on an agarose gel electrophoresis. 5 kbp 5 kbp 5 kb 5 kbp ----
Can you explain thie each of the statement given i dont really understand the dna recombinant is used as a molecular cloning and application for recombinant dna
10. Taq polymerase, a DNA polymerase derived from thermophilic bacteria, is used in polymerase chain reactions (PCR) in the laboratory. During PCR, Taq catalyzes DNA polymerization, similar to how it would in bacteria. A normal PCR cycle is as follows:1. Melting/Denaturing 95°C2. Primer Annealing 50°C3. Elongation of DNA (repeat 20–30 cycles) 72°C Which of the following conditions likely describes the living environment of Taq bacteria?(A) Freshwater with acidic pH(B) Hydrothermal vents reaching temperatures between 70– 75°C(C) Hot springs of 40°C(D) Tide pools with high salinity
1. What are restriction enzymes and what do they do? 2. Complete the chart Original DNA sequence 5' TGA CCA CTC GAG CAT AAC GAG TCG CTC 3° Write the complementary strand's sequence * remember it will be opposite directionality (the number at the ends are different) * pairs are A-T and C-G |Copy and paste your double stranded DNA into this box. You are going to use the restriction enzyme Xhol. Mark the cut site by changing the color (highlight or change text color) of one double-stranded piece * it cuts between the C and the T (cut shown as slash symbol): 5’ C/TC GAG 3' * remember it cuts BOTH strands (the restriction site is palindromic). Hint: read each strand in the 5'à 3' directions to find the cut site
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13. Technique whereby inserting DNA into a clone is accomplished using two different restriction enzymes Group of answer choices Primer Walking Positional Cloning Directional Cloning Chromosome Walking