A plasmid was cleaved with several restriction enzymes, individually and in combinations. The following fragment sizes (base pairs) were determined by agarose gel electrophoresis. Eco RI Aval Pvu || Pst | 4363 2182 4363 4363 2938 3609 3722 2182 Eco RI - Avo I Eco RI - Pst I Ava | - Pvu II Aval - Pst | Pvu II - Pst I 2820 Eco RI - Pvu II 2297 Make a restriction map based on this data. Note: There may be some slight discrepancy in summing up the total base pairs. Indicate the distances be- tween sites. Why is only one band detected in the Avo I - Pst I co-digest? 1425 754 641 1543 2066
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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