For your research, you set up a.PCR and put it into the thermocycler. While you are cleaning up, you notice that you added an extra ingredient...oops! The extra ingredient is a known inhibitor of primase. How would you expect this to affect your PCR results? Talk about what would still function and what would not during this PCR What would be in your tube at the end of PCR?

For your research, you set up a.PCR and put it into the thermocycler. While you are cleaning up, you notice that you added an extra ingredient...oops! The extra ingredient is a known inhibitor of primase. How would you expect this to affect your PCR results? Talk about what would still function and what would not during this PCR What would be in your tube at the end of PCR?

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 10SQ

See similar textbooks

Similar questions

What is the difference between reverse transcriptase PCR(RT-PCR) and standard PCR? For what purpose would youuse RT-PCR?
1. Why is each of the following reagents required to be in the PCR reaction tube? Primers, Taq DNA polymerase, nucleotide mix, reaction buffer 2. Imagine that you forgot to add nucleotides to your PCR master mix. How would this affect the outcome of your PCR reactions? a) If you made the mistake described in the previous question, which control would give you unexpected results? (In other words, if you forgot to add nucleotides, which control reaction tube would have results that are different that they would be if you had prepared them correctly?) 3. If you found that there was DNA amplification in your negative control tube, what could be an explanation for that result?
Please help me, this is from practice, double check your answers, previous tutors got it wrong
You are working in a molecular biology laboratory and are having challenges with your PCR. You decide to double-check the PCR protocol programmed into the thermal cycler and discover that the annealing temperature was programmed to be 65 °C instead of 50 °C, as you had intended. What effects would this mistake have on the PCR reaction? Suggest potential solution(s).
1. What happens in the denaturing step of PCR? A. What happens during the annealing step of PCR? B. What happens during the extension step of PCR? C. What temperatures is associated with each PCR step? D. Why is it necessary to have a primer on each side of the DNA segment to be amplified? (Hint: draw it out to see what happens with only one primer). E. How did the Taq DNA polymerase acquire its name? F. Why are there nucleotides (A, T, G, C) in the master mix? What are the other components of the master mix and what are their functions?
1. You decide to perform dideoxy sequencing on a PCR product. You add the appropriate 32P- labeled primer, DNA polymerase, DNA template (the PCR product), buffer, DNTP mix, and a small amount of one of the four ddNTPs to four reaction tubes. You run the reactions in the thermal cycler, load each reaction into a separate lane of a polyacrylamide gel, and separate the products by gel electrophoresis. In the figure below, the lanes are labeled according to the ddNTP added. ddATP ddTTP ddCTP ddGTP - Lane 1 2 3 4 a) In lane 5, draw what you would expect to see if you prepared a reaction using a nucleotide mix containing only DATP, DTTP, dCIP, and dGTP. b) In lane 6, draw what you would expect to see if you prepared a reaction using a nucleotide mix containing only ddATP, DTTP, dCIP, dGTP. ||
Describe PCR. Give a real world example of when PCR may be used in the lab to solve a problem. Do a little research if nothing comes into your mind. For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). B I US Paragraph Arial x² X₂ ¶ Ⓡ ||| ||| 冈 A v 880 V Ix % $1 田田田由用

A student carries out PCR using the following steps: step 1: 94C for 1 minuteStep 2: 60C for 30seconds Step 3: 72C for 30 seconds The student is examining a 1000-bp DNA fragment as part of their research project. If the limit of detection of this molecule 9 x 108 molecules, what is the minimum number of PCR cycles you would have to run to detect PCR product generated from a single molecule of the template? 2. Two double-stranded fragments of DNA are exactly the same length. At 89 C, fragment A has completely denatured which means the two strands have separated. At that temperature, fragment B is still double-stranded. How might these fragments differ to result in different denaturation temperatures?
1. Explain what primers are and what purpose they serve in a PCR reaction. Explain the main steps and temperatures of one PCR cycle. 2. Explain the purposes of the following components of gel electrophoresis: agarose, loading buffer, DNA ladder/marker, electrode buffer, electrodes. 3. Explain the expected results of the Alu sequence at PV92 that we tested, in terms of numbers of bands and their sizes. A labeled drawing would suffice.
1. Primers come at a concentration of 200 pmol//ul (uM). You want the final concentration of your primers to be 0.2 uM. How would you dilute the primers in water so that you can add 1 ul in a PCR mix with a total volume of 50ul?
The Lab technician was responsible for evaluating a genetic disorders by PCR. The technician programmed the termocylcler and left for the weekend. After two cycles, a power failure occured and the PCR reaction stopped. One of his colleque realized that and restart to thermocycler again. However, he did not anything know about PCR and asked you what to do: After running PCR products there was no band on the gel. The person decided to change one of the temperatures in the second cycle. Which temperature can be changed and explain why?
Can you help me with this question, please? What are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a. human error is reduced as there are fewer human interactions with the samples b. you can visualize the results as the process is running c. samples can be compared as to the amount of template DNA in the original sample d. more samples can be run in a day by one person